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Literature summary extracted from

  • Endo, S.; Matsunaga, T.; Mamiya, H.; Hara, A.; Kitade, Y.; Tajima, K.; El-Kabbani, O.
    Characterization of a rat NADPH-dependent aldo-keto reductase (AKR1B13) induced by oxidative stress (2009), Chem. Biol. Interact., 178, 151-157.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.21 additional information gene expression in YPEN-1 and NRK cells is up-regulated by treatments with submicromolar concentrations of hydrogen peroxide and 1,4-naphthoquinone, but not with fibroblast growth factor-1, fibroblast growth factor-2, 5alpha-dihydrotestosterone and 17beta-estradiol Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.21 expressed in Escherichia coli and in YPEN-1 and NRK cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 3',3'',5',5''-tetrabromophenolphthalein less than 25% inhibition at 0.1 mM Rattus norvegicus
1.1.1.21 Barbital less than 25% inhibition at 0.5 mM Rattus norvegicus
1.1.1.21 benzbromarone
-
 Rattus norvegicus
1.1.1.21 daidzein
-
 Rattus norvegicus
1.1.1.21 Diphenic acid less than 25% inhibition at 0.5 mM Rattus norvegicus
1.1.1.21 fidarestat
-
 Rattus norvegicus
1.1.1.21 Hexestrol
-
 Rattus norvegicus
1.1.1.21 indomethacin less than 25% inhibition at 0.1 mM Rattus norvegicus
1.1.1.21 Medroxyprogesterone acetate less than 25% inhibition at 0.01 mM Rattus norvegicus
1.1.1.21 minalrestat
-
 Rattus norvegicus
1.1.1.21 additional information the enzyme activity is not affected by 0.1 mM Wy14643 Rattus norvegicus
1.1.1.21 myricetin
-
 Rattus norvegicus
1.1.1.21 quercetin
-
 Rattus norvegicus
1.1.1.21 sorbinil
-
 Rattus norvegicus
1.1.1.21 Tolrestat
-
 Rattus norvegicus
1.1.1.21 Valproate less than 25% inhibition at 0.5 mM Rattus norvegicus
1.1.1.21 zearalenone
-
 Rattus norvegicus
1.1.1.21 zopolrestat
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.0035
-
 4-nitrobenzaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.005
-
 1-nonanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.0055
-
 1-decanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.0057
-
 16-ketoestrone in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.0065
-
 2,3-heptanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.0095
-
 21-Dehydrocortisol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.01
-
 2-decenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.012
-
 Pyridine-3-aldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.014
-
 2,3-Hexanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.014
-
 1-octanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.015
-
 1-butanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.016
-
 Phenylglyoxal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.017
-
 2-nonenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.02
-
 Pyridine-4-aldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.023
-
 1-hexanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.03
-
 4-hydroxynonenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.048
-
 2,4-decadienal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.052
-
 2-phenyl-2-propenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.056
-
 phenyl-1,2-propanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.063
-
 benzaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.064
-
 2,4-nonadienal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.069
-
 isatin in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.07
-
 3-deoxyglucosone in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.073
-
 D-lactoaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.075
-
 2-hexenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.082
-
 1-Decanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.12
-
 acrolein in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 2,3-Pentanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 (1S)-Camphorquinone in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.26
-
 methylglyoxal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.3
-
 1-propanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.31
-
 3,4-hexanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.68
-
 2,3-Butanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.74
-
 1-nonanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.78
-
 2-phenyl-1-propanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.97
-
 1-Hexanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 1.28
-
 Glyoxal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 1.5
-
 glycoaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 2
-
 DL-glyceraldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 2.1
-
 benzyl alcohol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 63
-
 1-butanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 35000
-
 gel filtration Rattus norvegicus
1.1.1.21 36000
-
 SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21 Q-Sepharose column chromatography and Sephadex G-100 gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 adrenal gland
-
 Rattus norvegicus
-
1.1.1.21 colon
-
 Rattus norvegicus
-
1.1.1.21 endothelial cell
-
 Rattus norvegicus
-
1.1.1.21 fibroblast
-
 Rattus norvegicus
-
1.1.1.21 heart
-
 Rattus norvegicus
-
1.1.1.21 kidney
-
 Rattus norvegicus
-
1.1.1.21 liver
-
 Rattus norvegicus
-
1.1.1.21 lung
-
 Rattus norvegicus
-
1.1.1.21 additional information the mRNA is detected in all rat tissues besides the brain, but contents in rat tissues other than the lung, stomach and adrenal gland are low Rattus norvegicus
-
1.1.1.21 ovary
-
 Rattus norvegicus
-
1.1.1.21 small intestine
-
 Rattus norvegicus
-
1.1.1.21 stomach
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 (1S)-camphorquinone + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 (S)-1-indanol + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 (S)-1-tetralol + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 1-butanal + NADPH + H+
-
 Rattus norvegicus 1-butanol + NADP+
-
 ?
1.1.1.21 1-decanal + NADPH + H+
-
 Rattus norvegicus 1-decanol + NADP+
-
 ?
1.1.1.21 1-hexanal + NADPH + H+
-
 Rattus norvegicus 1-hexanol + NADP+
-
 ?
1.1.1.21 1-nonanal + NADPH + H+
-
 Rattus norvegicus 1-nonanol + NADP+
-
 ?
1.1.1.21 1-propanal + NADPH + H+
-
 Rattus norvegicus 1-propanol + NADP+
-
 ?
1.1.1.21 16-ketoestrone + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2,3-butanedione + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2,3-heptanedione + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2,3-hexanedione + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2,3-pentanedione + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2,4-decadienal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2,4-nonadienal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2-decenal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2-hexenal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2-nonenal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 2-phenyl-2-propenal + NADPH + H+
-
 Rattus norvegicus 2-phenyl-1-propanol + NADP+
-
 ?
1.1.1.21 21-dehydrocortisol + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 3,4-hexanedione + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 3-deoxyglucosone + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 4-hydroxynonenal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 4-nitrobenzaldehyde + NADPH + H+
-
 Rattus norvegicus 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.21 acrolein + NADPH + H+
-
 Rattus norvegicus 2-propen-1-ol + NADP+
-
 ?
1.1.1.21 benzaldehyde + NADPH + H+
-
 Rattus norvegicus benzyl alcohol + NADP+
-
 ?
1.1.1.21 D-lactoaldehyde + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 DL-glyceraldehyde + NADPH + H+
-
 Rattus norvegicus glycerol + NADP+
-
 ?
1.1.1.21 glycoaldehyde + NADPH + H+
-
 Rattus norvegicus ethylene glycol + NADP+
-
 ?
1.1.1.21 glyoxal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 isatin + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 methylglyoxal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 additional information exhibits low NADP+-linked dehydrogenase activity towards aliphatic and aromatic alcohols Rattus norvegicus ?
-
 ?
1.1.1.21 additional information no NADH-linked reductase activity is observed Rattus norvegicus ?
-
 ?
1.1.1.21 octanal + NADPH + H+
-
 Rattus norvegicus 1-octanol + NADP+
-
 ?
1.1.1.21 phenyl-1,2-propanedione + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 phenylglyoxal + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 pyridine-3-aldehyde + NADPH + H+
-
 Rattus norvegicus 3-pyridinemethanol + NADP+
-
 ?
1.1.1.21 pyridine-3-methanol + NADPH + H+
-
 Rattus norvegicus ?
-
 ?
1.1.1.21 pyridine-4-aldehyde + NADPH + H+
-
 Rattus norvegicus 4-hydroxypyridine + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.21 AKR1B13
-
 Rattus norvegicus
1.1.1.21 aldo-keto reductase
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.21 0.002
-
 Pyridine-3-methanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.003
-
 (S)-1-tetralol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.007
-
 (S)-1-indanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.012
-
 2-phenyl-1-propanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.023
-
 1-Decanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.0383
-
 benzyl alcohol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.062
-
 1-nonanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.068
-
 1-Hexanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.13
-
 1-propanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.137
-
 1-butanol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.145
-
 2-decenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.155
-
 2-nonenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.183
-
 4-hydroxynonenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.183
-
 16-ketoestrone in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 Glyoxal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 glycoaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 3-deoxyglucosone in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 2,4-decadienal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 21-Dehydrocortisol in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.2
-
 2,4-nonadienal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.233
-
 3,4-hexanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.25
-
 2,3-Hexanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.27
-
 acrolein in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.283
-
 isatin in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.283
-
 D-lactoaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.333
-
 2,3-Butanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.333
-
 (1S)-Camphorquinone in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.35
-
 1-decanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.383
-
 2,3-Pentanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.383
-
 phenyl-1,2-propanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.42
-
 benzaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.42
-
 Phenylglyoxal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.43
-
 methylglyoxal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.43
-
 2-phenyl-2-propenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.52
-
 Pyridine-3-aldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.57
-
 4-nitrobenzaldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.57
-
 1-nonanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.583
-
 DL-glyceraldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.6
-
 2,3-heptanedione in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.63
-
 Pyridine-4-aldehyde in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.67
-
 2-hexenal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.67
-
 1-butanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.7
-
 1-octanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus
1.1.1.21 0.75
-
 1-hexanal in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.21 6 8 the NADPH-linked pyridine-3-aldehyde reductase activity is gradually increased by decreasing pH from 8.0 to 6.0 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADPH strict coenzyme specificity for NADPH Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.21 0.00003
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus (4-oxo-3-{[5-(trifluoromethyl)-1,3-benzothiazol-2-yl]methyl}-3,4-dihydrophthalazin-1-yl)acetic acid
1.1.1.21 0.0006
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus minalrestat
1.1.1.21 0.0008
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus Tolrestat
1.1.1.21 0.0008
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus benzbromarone
1.1.1.21 0.0032
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus Hexestrol
1.1.1.21 0.0068
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus myricetin
1.1.1.21 0.0085
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus fidarestat
1.1.1.21 0.01
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus daidzein
1.1.1.21 0.025
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus zearalenone
1.1.1.21 0.03
-
 in 0.1 M potassium phosphate, pH 7.4 Rattus norvegicus sorbinil