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Literature summary extracted from

  • Giedraityte, G.; Kalediene, L.
    Catechol 1,2-dioxygenase from alpha-naphthol degrading thermophilic Geobacillus sp. strain: Purification and properties (2009), Cent. Eur. J. Biol., 4, 68-73.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 AgNO3 0.1 mM, 10 min, complete inhibition Geobacillus sp.
1.13.11.1 CuSO4 0.1 mM, 10 min, complete inhibition Geobacillus sp.
1.13.11.1 EDTA 1 mM, 10 min, 20% inhibition Geobacillus sp.
1.13.11.1 FeSO4 0.1 mM, 10 min, 12% inhibition Geobacillus sp.
1.13.11.1 H2O2 0.1 mM, 10 min, 98% inhibition Geobacillus sp.
1.13.11.1 mercaptoethanol 0.1 mM, 10 min, 98% inhibition Geobacillus sp.
1.13.11.1 o-phenanthroline 0.05 mM, 10 min, 3% inhibition Geobacillus sp.
1.13.11.1 streptomycin sulfate 5%, 10 min, 80% inhibition Geobacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.029
-
 catechol
-
 Geobacillus sp.
1.13.11.1 0.286
-
 3-methylcatechol
-
 Geobacillus sp.
1.13.11.1 0.4
-
 4-methylcatechol
-
 Geobacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 96000
-
 SDS-PAGE Geobacillus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Geobacillus sp.
-
-
-
1.13.11.1 Geobacillus sp. G27
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 about 18fold after ammonium sulfate precipitation, DEAE-celullose, Sephadex G-150 and hydroxylapatite chromatographie Geobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 7.42
-
-
 Geobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. ?
-
 ?
1.13.11.1 3-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. G27 ?
-
 ?
1.13.11.1 4-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. ?
-
 ?
1.13.11.1 4-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. G27 ?
-
 ?
1.13.11.1 catechol + O2 intradiol, assay at 60°C, pH 7.0 Geobacillus sp. cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2 intradiol, assay at 60°C, pH 7.0 Geobacillus sp. G27 cis,cis-muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.1 C1,2O
-
 Geobacillus sp.
1.13.11.1 catechol 1,2-dioxygenase
-
 Geobacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 60
-
-
 Geobacillus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.1 30 90 only 3% enzyme activity lost at 50-70°C, at 80°C 22% lose of 22% of enzyme activity, below 50°C and 70°C rapidly decreased enzyme activity Geobacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.1 4
-
 2 month: 100% enzyme activity left Geobacillus sp.
1.13.11.1 60
-
 half-life: 40 min Geobacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7
-
-
 Geobacillus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.1 5 11 45% of specific activity in pH range from 8-10.5 Geobacillus sp.