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Literature summary extracted from

  • Inouye, S.
    Firefly luciferase: an adenylate-forming enzyme for multicatalytic functions (2010), Cell. Mol. Life Sci., 67, 387-404.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.7 ATP essential for luminescence reaction Luciola cruciata
1.13.12.7 ATP essential for luminescence reaction Pyrophorus plagiophthalamus
1.13.12.7 ATP essential for luminescence reaction, initiates luminescence reaction Photinus pyralis
1.13.12.7 CoA prevents rapid decay of light emission Photinus pyralis
1.13.12.7 diphosphate stimulates light production under some conditions Photinus pyralis
1.13.12.7 molecular oxygen essential for luminescence reaction Photinus pyralis
1.13.12.7 molecular oxygen essential for luminescence reaction Luciola cruciata
1.13.12.7 molecular oxygen essential for luminescence reaction Pyrophorus plagiophthalamus
1.13.12.7 Triphosphate stimulates light production under some conditions Photinus pyralis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.7 expression in Escherichia coli Photinus pyralis
6.2.1.3 expression in Escherichia coli Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.12.7
-
Photinus pyralis

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.7 additional information chimeric protein with fatty acyl-CoA synthetase, EC 6.2.1.3, 4% of luminescence activity from wild-tpe luciferase activity Photinus pyralis
1.13.12.7 S286N red bioluminescence Luciola cruciata
6.2.1.3 C343S no luminescence enhancement Agrypnus binodulus
6.2.1.3 C343S/G344T no luminescence enhancement Agrypnus binodulus
6.2.1.3 C343S/G344T/L345S increase of luminescence activity to 100% Agrypnus binodulus
6.2.1.3 C343S/L345S increase of luminescence activity Agrypnus binodulus
6.2.1.3 G344T no luminescence enhancement Agrypnus binodulus
6.2.1.3 G344T/L345S increase of luminescence activity to 27% Agrypnus binodulus
6.2.1.3 L216R no luminescence enhancement Agrypnus binodulus
6.2.1.3 L345S enhancement of luminescence Agrypnus binodulus
6.2.1.3 additional information chimeric protein with firefly luciferase, EC 1.13.12.7, 4% of luminescence activity from Photinus pyralis luciferase activity Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 2-phenylbenzothiazole
-
Photinus pyralis
1.13.12.7 5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine competitive inhibitor Photinus pyralis
1.13.12.7 AMP
-
Photinus pyralis
1.13.12.7 arachidonic acid concentration above 10 microM Photinus pyralis
1.13.12.7 ATP
-
Photinus pyralis
1.13.12.7 bromoform
-
Photinus pyralis
1.13.12.7 dehydroluciferin
-
Luciola cruciata
1.13.12.7 dehydroluciferin
-
Photinus pyralis
1.13.12.7 dehydroluciferyl adenylate
-
Luciola cruciata
1.13.12.7 dehydroluciferyl adenylate
-
Photinus pyralis
1.13.12.7 diphosphate depending on conditions Photinus pyralis
1.13.12.7 Halothane non-competitive inhibitor Photinus pyralis
1.13.12.7 L-luciferin
-
Luciola cruciata
1.13.12.7 L-luciferin
-
Photinus pyralis
1.13.12.7 linoleic acid concentration above 10 microM Photinus pyralis
1.13.12.7 lipoic acid
-
Photinus pyralis
1.13.12.7 myristic acid
-
Photinus pyralis
1.13.12.7 N-pyridin-2-ylbenzamide
-
Photinus pyralis
1.13.12.7 oleic acid concentration above 10 microM Photinus pyralis
1.13.12.7 oxyluciferin
-
Luciola cruciata
1.13.12.7 oxyluciferin
-
Photinus pyralis
1.13.12.7 pifithrin-alpha 20 microM, above 95% inhibition Photinus pyralis
6.2.1.3 arachidonic acid concentration above 10 microM Drosophila melanogaster
6.2.1.3 linoleic acid concentration above 10 microM Drosophila melanogaster
6.2.1.3 oleic acid concentration above 10 microM Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.7 0.0024
-
D-luciferin native luciferase Photinus pyralis
1.13.12.7 0.0024
-
D-luciferin native luciferase Luciola cruciata
1.13.12.7 0.0047
-
D-luciferin recombinant luciferase Photinus pyralis
1.13.12.7 0.0047
-
D-luciferin recombinant luciferase Luciola cruciata
1.13.12.7 0.00741
-
lauric acid
-
Photinus pyralis
1.13.12.7 0.0136
-
linoleic acid
-
Photinus pyralis
1.13.12.7 0.015
-
D-luciferin
-
Photinus pyralis
1.13.12.7 0.0163
-
lauric acid
-
Luciola cruciata
1.13.12.7 0.16
-
ATP
-
Photinus pyralis
6.2.1.3 0.00168
-
lauric acid
-
Drosophila melanogaster
6.2.1.3 0.00188
-
linoleic acid
-
Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Mg2+ essential Photinus pyralis
1.13.12.7 Mg2+ essential Luciola cruciata
1.13.12.7 Mg2+ essential Pyrophorus plagiophthalamus

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Aquatica lateralis Q01158
-
-
1.13.12.7 Cratomorphus distinctus Q5USC8
-
-
1.13.12.7 Hotaria unmunsana Q8T6U3
-
-
1.13.12.7 Lampyris noctiluca Q27688
-
-
1.13.12.7 Lampyris turkestanicus Q5UFR2
-
-
1.13.12.7 Lampyroidea maculata Q1WLP6
-
-
1.13.12.7 Luciola cruciata P13129
-
-
1.13.12.7 Luciola mingrelica Q26304
-
-
1.13.12.7 Luciola parvula Q25118
-
-
1.13.12.7 Photinus pyralis P08659
-
-
1.13.12.7 Photuris pensylvanica Q27757
-
-
1.13.12.7 Phrixothrix hirtus Q9U4U7
-
-
1.13.12.7 Phrixothrix vivianii Q9U4U8
-
-
1.13.12.7 Pyrearinus termitilluminans AF116843
-
-
1.13.12.7 Pyrocoelia miyako Q26076
-
-
1.13.12.7 Pyrocoelia rufa Q9GPF9
-
-
1.13.12.7 Pyrophorus mellifluus Q717B6
-
-
1.13.12.7 Pyrophorus plagiophthalamus Q718F0
-
-
6.2.1.3 Agrypnus binodulus
-
-
-
6.2.1.3 Drosophila melanogaster Q9VCC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC Luciola cruciata
1.13.12.7 ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC Aquatica lateralis
1.13.12.7 gel filtration, affinity chromatography Photinus pyralis
6.2.1.3
-
Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.7 additional information
-
lower luminescence activity when another substrate than D-luciferin is used: only 10% luminescence activity with 6'-amino-D-luciferin, only 5% luminescence activity with 4'-methyl-D-luciferin, only 7% luminescence activity with D-quinolylluciferin, only 1.5% luminescence activity with D-naphthylluciferin, only 16% luminescence activity with D-5,5-dimethylluciferyl-adenylate Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 4'-methyl-D-luciferin + ATP + O2
-
Photinus pyralis ?
-
?
1.13.12.7 5-methylluciferin + ATP + O2
-
Photinus pyralis ?
-
?
1.13.12.7 6'-amino-D-luciferin + ATP + O2
-
Photinus pyralis ?
-
?
1.13.12.7 D-5,5-dimethylluciferyl-adenylate + O2 yellow-green color of luminescence Photinus pyralis ?
-
?
1.13.12.7 D-5,5-dimethylluciferyl-adenylate + O2 + ATP red color of luminescence Pyrophorus plagiophthalamus ?
-
?
1.13.12.7 D-luciferin + ATP + O2
-
Luciola cruciata luciferyl-adenylate + diphosphate
-
r
1.13.12.7 D-luciferin + ATP + O2
-
Photinus pyralis luciferyl-adenylate + diphosphate + H2O + hv
-
r
1.13.12.7 D-naphthylluciferin + ATP + O2
-
Photinus pyralis ?
-
?
1.13.12.7 D-quinolylluciferin + ATP + O2
-
Photinus pyralis ?
-
?
1.13.12.7 dehydroluciferin + ATP
-
Photinus pyralis dehydroluciferyl-AMP + diphosphate
-
r
1.13.12.7 dehydroluciferin + ATP
-
Luciola cruciata dehydroluciferyl-AMP + diphosphate
-
r
1.13.12.7 lauric acid + ATP + CoA
-
Photinus pyralis lauroyl-CoA + AMP + diphosphate
-
?
1.13.12.7 lauric acid + ATP + CoA
-
Luciola cruciata lauroyl-CoA + AMP + diphosphate
-
?
1.13.12.7 linoleic acid + ATP + CoA linoleic acid concentration above 10 microM has an inhibitory effect Photinus pyralis linoleoyl-CoA + AMP + diphosphate
-
?
1.13.12.7 luciferyl-adenylate + O2
-
Photinus pyralis oxyluciferin + AMP + CO2 + hv
-
ir
1.13.12.7 luciferyl-adenylate + O2
-
Luciola cruciata oxyluciferin + AMP + CO2 + hv
-
ir
1.13.12.7 R-COOH + ATP
-
Photinus pyralis R-CO-AMP + diphosphate
-
r
1.13.12.7 R-COOH + ATP
-
Luciola cruciata R-CO-AMP + diphosphate
-
r
6.2.1.3 lauric acid
-
Drosophila melanogaster ?
-
?
6.2.1.3 linoleic acid + ATP + CoA
-
Drosophila melanogaster linoleoyl-CoA + AMP + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.7 firefly luciferase
-
Photinus pyralis
1.13.12.7 firefly luciferase
-
Luciola cruciata
1.13.12.7 luciferase
-
Cratomorphus distinctus
1.13.12.7 luciferase
-
Phrixothrix vivianii
1.13.12.7 luciferase
-
Phrixothrix hirtus
1.13.12.7 luciferase
-
Lampyris turkestanicus
1.13.12.7 luciferase
-
Luciola mingrelica
1.13.12.7 luciferase
-
Pyrophorus plagiophthalamus
1.13.12.7 luciferase
-
Aquatica lateralis
1.13.12.7 luciferase
-
Photuris pensylvanica
1.13.12.7 luciferase
-
Pyrocoelia miyako
1.13.12.7 luciferase
-
Pyrocoelia rufa
1.13.12.7 luciferase
-
Luciola parvula
1.13.12.7 luciferase
-
Hotaria unmunsana
1.13.12.7 luciferase
-
Lampyris noctiluca
1.13.12.7 luciferase
-
Lampyroidea maculata
1.13.12.7 luciferase
-
Pyrophorus mellifluus
1.13.12.7 luciferase
-
Pyrearinus termitilluminans
6.2.1.3 Abll
-
Agrypnus binodulus
6.2.1.3 long fatty acyl-CoA synthetase
-
Drosophila melanogaster
6.2.1.3 luciferase-like gene
-
Agrypnus binodulus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 5.4
-
red emitted light Photinus pyralis
1.13.12.7 7.6
-
yellow-green emitted light Photinus pyralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.7 7.5 8.5
-
Photinus pyralis
6.2.1.3 7.5 8.5
-
Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.7 ATP
-
Photinus pyralis
1.13.12.7 CoA
-
Photinus pyralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.12.7 additional information
-
dehydroluciferyl adenylate value between 0.0000025 - 0.00125 mM Luciola cruciata
1.13.12.7 additional information
-
5'-O-[hydroxy({[(2Z)-2-(6-oxo-1,3-benzothiazol-2(6H)-ylidene)-2,3-dihydro-1,3-thiazol-4-yl]carbonyl}oxy)phosphoryl]adenosine value between 0.0000025-0.00125 mM Photinus pyralis
1.13.12.7 additional information
-
oxyluciferin value between 0.0005-0.002 mM Photinus pyralis
1.13.12.7 additional information
-
oxyluciferin value between 0.0005-0.002 mM Luciola cruciata
1.13.12.7 additional information
-
L-luciferin value between 0.003-0.004 mM Luciola cruciata
1.13.12.7 0.000026
-
lipoic acid
-
Photinus pyralis
1.13.12.7 0.000034
-
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine for D-luciferin Photinus pyralis
1.13.12.7 0.000041
-
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine for MgATP2- Photinus pyralis
1.13.12.7 0.00034
-
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine for luciferyl adenylate Photinus pyralis
1.13.12.7 0.00053
-
myristic acid
-
Photinus pyralis
1.13.12.7 0.003 0.004 L-luciferin
-
Photinus pyralis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.12.7 0.00005
-
-
Photinus pyralis lipoic acid
1.13.12.7 0.000069
-
-
Photinus pyralis N-pyridin-2-ylbenzamide
1.13.12.7 0.00068
-
-
Photinus pyralis myristic acid

General Information

EC Number General Information Comment Organism
1.13.12.7 physiological function fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme Photinus pyralis
1.13.12.7 physiological function fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme Luciola cruciata