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Literature summary extracted from

  • Khan, F.S.; Fujioka, M.; Datta, P.; Fernandes-Alnemri, T.; Jaynes, J.B.; Alnemri, E.S.
    The interaction of DIAP1 with dOmi/HtrA2 regulates cell death in Drosophila (2008), Cell Death Differ., 15, 1073-1083.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.108 constructs encoding C-terminal His6-tagged full-length dOmi or truncated mutants are generated using the pET-21a and the pVL-1393 transfer vector, proteins are overexpressed in Escherichia coli BL21DE3 and Sf9 insect cells, respectively Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.108 cytosol released into the cytosol during apoptosis Drosophila melanogaster 5829
-
3.4.21.108 mitochondrial intermembrane space
-
 Drosophila melanogaster 5758
-
3.4.21.108 mitochondrion
-
 Drosophila melanogaster 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.108 33000
-
 dOmi-S, determined by SDS-PAGE and Western Blot analysis Drosophila melanogaster
3.4.21.108 35000
-
 dOmi-L, determined by SDS-PAGE and Western Blot analysis Drosophila melanogaster
3.4.21.108 38000
-
 unprocessed dOmi, determined by SDS-PAGE and Western Blot analysis Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.108 additional information Drosophila melanogaster cleavage of DIAP1 between the BIR1 and BIR2 domain and further degradation of BIR1 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.108 Drosophila melanogaster Q9VFJ3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.108 phosphoprotein
-
 Drosophila melanogaster
3.4.21.108 ubiquitination
-
 Drosophila melanogaster

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.108 using TALON agarose Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.108 eye eye disc Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.108 additional information cleavage of DIAP1 between the BIR1 and BIR2 domain and further degradation of BIR1 Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.108 dOmi/HtrA2 two isoforms, dOmi-L, dOmi-S Drosophila melanogaster
3.4.21.108 Omi/HtrA2
-
 Drosophila melanogaster
3.4.21.108 Omi/HtrA2 serine protease
-
 Drosophila melanogaster