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Literature summary extracted from

  • Wang, S.L.; Peng, J.H.; Liang, T.W.; Liu, K.C.
    Purification and characterization of a chitosanase from Serratia marcescens TKU011 (2008), Carbohydr. Res., 343, 1316-1323.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.132 additional information chitosanase is activated after keeping at 4°C for 10 days, in the presence of 25% (v/v) toluene, acetonitrile, and butanol Serratia marcescens

Application

EC Number Application Comment Organism
3.2.1.132 food industry usage of strain TKU011 on the microbial reclamation of food processing wastes such as shrimp shell wastes for the production of chitosanase Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Cu2+ 5 mM inhibits by 92% at 37°C for 30 min Serratia marcescens
3.2.1.132 EDTA 5 mM completely inhibits at 37°C for 30 min Serratia marcescens
3.2.1.132 Fe2+ 5 mM completely inhibits at 37°C for 30 min Serratia marcescens
3.2.1.132 Mn2+ 5 mM completely inhibits at 37°C for 30 min Serratia marcescens
3.2.1.132 additional information 5 mM Ba2+, Mg2+ and Ca2+ have little effect on activity at 37°C for 30 min. After keeping at 25°C for 10 days, the chitosanase is inactivated in the presence of 25% (v/v) ethanol and ethyl ether Serratia marcescens
3.2.1.132 PMSF 5 mM inhibits by 73% at 37°C for 30 min Serratia marcescens
3.2.1.132 Zn2+ 5 mM inhibits by 49% at 37°C for 30 min Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 18000
-
gel filtration Serratia marcescens
3.2.1.132 18780
-
sequence analysis Serratia marcescens
3.2.1.132 21000
-
1 * 21000, SDS-PAGE Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Serratia marcescens
-
-
-
3.2.1.132 Serratia marcescens TKU011
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 by a combination of ion-exchange and gel-filtration, 50fold with an overall activity yield of 24% Serratia marcescens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 0.002
-
culture supernatant Serratia marcescens
3.2.1.132 7
-
50fold purified enzyme Serratia marcescens

Storage Stability

EC Number Storage Stability Organism
3.2.1.132 25°C, the chitosanase is inactivated in the presence of 25% (v/v) ethanol and ethyl ether, 10 days Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 60-95% degree of deacetylation Serratia marcescens ?
-
?
3.2.1.132 chitosan + H2O activity decreases with the degree of deacetylation of chitosan. The enzyme hydrolyzes chitosan with 60-95% degree of deacetylation Serratia marcescens TKU011 ?
-
?
3.2.1.132 additional information no activity against colloidal chitin, chitin (a-type) and chitin (b-type). The enzyme has antioxidant activity Serratia marcescens ?
-
?
3.2.1.132 additional information no activity against colloidal chitin, chitin (a-type) and chitin (b-type). The enzyme has antioxidant activity Serratia marcescens TKU011 ?
-
?
3.2.1.132 shrimp-shell powder + H2O sole carbon/nitrogen source Serratia marcescens ?
-
?
3.2.1.132 shrimp-shell powder + H2O sole carbon/nitrogen source Serratia marcescens TKU011 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 monomer 1 * 21000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
-
Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 25 50 maintains more than 90% of its initial activity between 25 and 50°C, but is rapidly inactivated retaining only 60% of its initial activity at 60°C, and is completely inactivated at greater than 70°C Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 5
-
-
Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 4 8
-
Serratia marcescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.132 Serratia marcescens sequence analysis
-
7.11