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Literature summary extracted from

  • Santini, S.; Monchois, V.; Mouz, N.; Sigoillot, C.; Rousselle, T.; Claverie, J.; Abergel, C.
    Structural characterization of CA1462, the Candida albicans thiamine pyrophosphokinase (2008), BMC Struct. Biol., 8, 33.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.2 gene CA1462, expression in Escherichia coli Candida albicans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.2 purified recombinant enzyme in complex with ATP analogue AMP-PNP and thiamine, hanging drop vapor diffusion method, mixing of 500 nl of protein solution with 500 nl of reservoir solution containing 17.5-20% PEG 4000, 0.2 M MgCl2 , 0.1 M Tris, and 20% glycerol, pH 7.0-pH 7.5, and equilibration against 1 ml or reservoir solution, X-ray diffraction structure determination and analysis at 1.96-2.1 A resolution Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.2 Mg2+ ligands electronic density map and Mg2+ coordination structure, the delta-oxygen from D113 and D115 and one oxygen atom from each phosphate group of the thiamine-PNP. The last coordination of this Mg2+ involves an oxygen atom from the free inorganic phosphate, overview Candida albicans
2.7.6.2 additional information ligand binding structures, overview Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.2 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.2 recombinant CA1462 from Escherichia coli Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.2 0.000367
-
 recombinant enzyme Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.2 additional information thiamine binding site structure, overview Candida albicans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.2 dimer three-dimensional enzyme structure, modelling, overview Candida albicans

Synonyms

EC Number Synonyms Comment Organism
2.7.6.2 CA1462
-
 Candida albicans
2.7.6.2 thiamine pyrophosphokinase
-
 Candida albicans
2.7.6.2 TPK
-
 Candida albicans