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Literature summary extracted from

  • Rutten, L.; Ribot, C.; Trejo-Aguilar, B.; Woesten, H.A.; de Vries, R.P.
    A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol (2009), BMC Microbiol., 9, 166.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.12 derivatives of the expression vector pQE32 containing wild-type and mutated versions of ladA transformed to Escherichia coli M13 cells Aspergillus niger

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.12 modelling of LadA on human SDH identifies residues M70 and Y318 in LadA, that may explain the absence of activity on D-sorbitol Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.12 M70F almost complete loss of activity Aspergillus niger
1.1.1.12 M70F results in a nearly complete enzyme inactivation Aspergillus niger
1.1.1.12 Y318F results in a significant increase in affinity for D-sorbitol, xylitol and L-arabitol Aspergillus niger
1.1.1.12 Y318F increased activity for L-arabinitol and xylitol, increased affinity for D-sorbitol Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.12 0.056
-
 L-arabitol wild-type Aspergillus niger
1.1.1.12 0.056
-
 L-arabinitol wild-type, pH 9.6, 20°C Aspergillus niger
1.1.1.12 0.078
-
 L-arabitol mutant Y318F Aspergillus niger
1.1.1.12 0.078
-
 L-arabinitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
1.1.1.12 0.218
-
 xylitol mutant Y318F Aspergillus niger
1.1.1.12 0.218
-
 xylitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
1.1.1.12 0.25
-
 xylitol wild-type Aspergillus niger
1.1.1.12 0.25
-
 xylitol wild-type, pH 9.6, 20°C Aspergillus niger
1.1.1.12 0.868
-
 D-sorbitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
1.1.1.12 4.12
-
 D-sorbitol wild-type, pH 9.6, 20°C Aspergillus niger
1.1.1.12 4.122
-
 D-sorbitol wild-type Aspergillus niger
1.1.1.12 4.868
-
 D-sorbitol mutant Y318F Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.12 Aspergillus niger A2QAC0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.12 recombinant proteins purified using Ni-agarose Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.12 D-sorbitol + NAD+
-
 Aspergillus niger D-fructose + NADH + H+
-
 ?
1.1.1.12 D-sorbitol + NAD+ wild-type shows no activity on D-sorbitol, whereas mutant Y318F does Aspergillus niger ? + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Aspergillus niger L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabitol + NAD+
-
 Aspergillus niger L-xylulose + NADH + H+
-
 ?
1.1.1.12 additional information Y318 of LadA contributes significantly to the substrate specificity difference between LAD and xylitol dehydrogenase/D-sorbitol dehydrogenase Aspergillus niger ?
-
 ?
1.1.1.12 xylitol + NAD+
-
 Aspergillus niger D-xylulose + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.12 L-arabitol dehydrogenase
-
 Aspergillus niger
1.1.1.12 LAD
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.12 13.48
-
 D-sorbitol wild-type, pH 9.6, 20°C Aspergillus niger
1.1.1.12 13.88
-
 D-sorbitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
1.1.1.12 14.4
-
 L-arabinitol wild-type, pH 9.6, 20°C Aspergillus niger
1.1.1.12 19.67
-
 xylitol wild-type, pH 9.6, 20°C Aspergillus niger
1.1.1.12 30
-
 L-arabinitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
1.1.1.12 36.8
-
 xylitol mutant Y318F, pH 9.6, 20°C Aspergillus niger
1.1.1.12 809
-
 D-sorbitol wild-type Aspergillus niger
1.1.1.12 833
-
 D-sorbitol mutant Y318F Aspergillus niger
1.1.1.12 863
-
 L-arabitol wild-type Aspergillus niger
1.1.1.12 1180
-
 xylitol wild-type Aspergillus niger
1.1.1.12 1800
-
 L-arabitol mutant Y318F Aspergillus niger
1.1.1.12 2208
-
 xylitol mutant Y318F Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.12 NAD+
-
 Aspergillus niger