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Literature summary extracted from

  • Haibo, Z.; Yinglong, Z.; Feng, H.; Peiji, G.; Jiachuan, C.
    Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta (2009), Biotechnol. Lett., 31, 837-843.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 CuSO4 activates 113% at over 40 mM with substrate 3,6-dimethoxyphenol, and 46% at over 10 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
1.10.3.2 EDTA activates 10% at over 80 mM with substrate 3,6-dimethoxyphenol, and 50% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
1.10.3.2 MnSO4 activates 49% at over 80 mM with substrate 3,6-dimethoxyphenol, and 28% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
1.10.3.2 oxalic acid activates 42% at 2 mM with substrate 3,6-dimethoxyphenol, and 303% at 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 DTT complete inhibition at over 10 mM with substrate 3,6-dimethoxyphenol and at over 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
1.10.3.2 NaF complete inhibition at over 10 mM with substrates 3,6-dimethoxyphenol and 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
1.10.3.2 NaN3 complete inhibition at over 80 mM with substrate 3,6-dimethoxyphenol and at over 40 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.07
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.8, 25°C Trametes hirsuta
1.10.3.2 0.2
-
 2,6-dimethoxyphenol pH 4.8, 25°C Trametes hirsuta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ a copper enzyme Trametes hirsuta
1.10.3.2 additional information the enzyme is not affected by Cu2+, Mn2+, or EDTA Trametes hirsuta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 90000
-
 x * 90000, SDS-PAGE Trametes hirsuta

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Trametes hirsuta
-
 isolated from Mengshan Mountain in Shandong Province, China
-
1.10.3.2 Trametes hirsuta Ig-9 / CGMCC 2422
-
 isolated from Mengshan Mountain in Shandong Province, China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native enzyme 180fold by cold precipitation, ultrafiltration ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Trametes hirsuta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Trametes hirsuta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 360
-
 purified native enzyme Trametes hirsuta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 1,3,5-trihydroxybenzene + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 1,3,5-trihydroxybenzene + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 high activity Trametes hirsuta ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
1.10.3.2 3-aminobenzoic acid + O2 low activity Trametes hirsuta ?
-
 ?
1.10.3.2 alizarin red S + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 catechol + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 catechol + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
1.10.3.2 methyl red + O2
-
 Trametes hirsuta ?
-
 ?
1.10.3.2 additional information no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview Trametes hirsuta ?
-
 ?
1.10.3.2 additional information no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 90000, SDS-PAGE Trametes hirsuta

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 More the enzyme belongs to the multicopper oxidase family Trametes hirsuta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 85
-
 substrate 2,6-dimethoxyphenol Trametes hirsuta

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 25 90
-
 Trametes hirsuta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 65
-
 half-life is 5 h Trametes hirsuta
1.10.3.2 75
-
 half-life is 70 min Trametes hirsuta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 50
-
 2,6-dimethoxyphenol pH 4.8, 25°C Trametes hirsuta
1.10.3.2 197
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.8, 25°C Trametes hirsuta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.4 2.5
-
 Trametes hirsuta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2.2 5 activity range Trametes hirsuta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Trametes hirsuta isoelectric focussing
-
 4.3