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Literature summary extracted from

  • Bankar, S.B.; Bule, M.V.; Singhal, R.S.; Ananthanarayan, L.
    Glucose oxidase - an overview (2009), Biotechnol. Adv., 27, 489-501.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 diagnostics used in an automatic glucose assay kit in conjunction with catalase and chiefly in biosensors for the detection and estimation of glucose in industrial solutions and in body fluids such as blood and urine Aspergillus niger
1.1.3.4 food industry food and beverage additive, used for low alcohol wine production, used for glucose removal from dried egg, improvement of color, flavor, and shelf life of food materials, oxygen removal from fruit juices, canned beverages, and from mayonnaise to prevent rancidity, used as ingredient of toothpaste, for the production of gluconic acid, and as a food preservative Aspergillus niger
1.1.3.4 synthesis GOD is used as a commercial source of gluconic acid, which can be produced by the hydrolysis of delta-glucono-1, 5-lactone, the endproduct of GOD catalysis Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.4 expressed in Saccharomyces cerevisiae Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.4 4-chloromercuribenzoate
-
 Aspergillus niger
1.1.3.4 Ag+
-
 Aspergillus niger
1.1.3.4 Cu2+
-
 Aspergillus niger
1.1.3.4 dimedone
-
 Aspergillus niger
1.1.3.4 Hg2+
-
 Aspergillus niger
1.1.3.4 hydrazine
-
 Aspergillus niger
1.1.3.4 hydroxylamine
-
 Aspergillus niger
1.1.3.4 phenylhydrazine
-
 Aspergillus niger
1.1.3.4 sodium bisulfate
-
 Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 33
-
 beta-D-glucose
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.4 152000
-
 SDS-PAGE Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 ammonium sulfate precipitation, ion exchange chromatography, and gel filtration Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 mycelium
-
 Aspergillus niger
-

Storage Stability

EC Number Storage Stability Organism
1.1.3.4 -20°C, lyophilised enzyme, minimum of 6 months, remains stable Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 2-deoxy-D-glucose + O2 + H2O low GOD activity Aspergillus niger 2-deoxy-D-glucono-1,5-lactone + H2O2
-
 ?
1.1.3.4 beta-D-glucose + O2 + H2O GOD is highly specific for the beta-anomer of D-glucose Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ir
1.1.3.4 D-galactose + O2 + H2O low GOD activity Aspergillus niger ?
-
 ?
1.1.3.4 D-mannose + O2 + H2O low GOD activity Aspergillus niger ?
-
 ?
1.1.3.4 additional information alpha-D-glucose is not a suitable substrate Aspergillus niger ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 beta-D-glucose:oxygen 1-oxidoreductase
-
 Aspergillus niger
1.1.3.4 GOD
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.4 40 60
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.4 37
-
 GOD has half-life of approximately 30 min at 37°C, immobilized GOD is more effective for applications at 37°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.4 5 6
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.4 3.5 6.5
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.4 FAD
-
 Aspergillus niger