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Literature summary extracted from

  • Gao, F.; Courjean, O.; Mano, N.
    An improved glucose/O2 membrane-less biofuel cell through glucose oxidase purification (2009), Biosens. Bioelectron., 25, 356-361.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 biofuel production used in miniature membrane-less glucose/O2 biofuel cells Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.4 1.9
-
 beta-D-glucose purified enzyme under argon, in 20 mM phosphate buffer pH 7.4 at 37°C Aspergillus niger
1.1.3.4 2.5
-
 beta-D-glucose non-purified enzyme under argon, in 20 mM phosphate buffer pH 7.4 at 37°C Aspergillus niger
1.1.3.4 11.9
-
 beta-D-glucose purified enzyme under O2, in 20 mM phosphate buffer pH 7.4 at 37°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.4 HiTrap phenyl HP column chromatography and HiTrap Q FF column chromatography Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.4 GOX
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.4 FAD
-
 Aspergillus niger