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Literature summary extracted from

  • Tanaka, M.; Umemoto, Y.; Okamura, H.; Nakano, D.; Tamaru, Y.; Araki, T.
    Cloning and characterization of a beta-1,4-mannanase 5C possessing a family 27 carbohydrate-binding module from a marine bacterium, Vibrio sp. strain MA-138 (2009), Biosci. Biotechnol. Biochem., 73, 109-116.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 1 mM, more than 80% inhibition Vibrio sp.
3.2.1.78 Al3+ 1 mM, more than 80% inhibition Vibrio sp.
3.2.1.78 Cu2+ 1 mM, more than 80% inhibition Vibrio sp.
3.2.1.78 Hg2+ 1 mM, more than 80% inhibition Vibrio sp.
3.2.1.78 Pb2+ 1 mM, more than 80% inhibition Vibrio sp.
3.2.1.78 Zn2+ 1 mM, more than 80% inhibition Vibrio sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.78 additional information
-
additional information Km value for konjac glucomannan 0.6 mg/ml Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 70000
-
gel fitlration Vibrio sp.
3.2.1.78 73000
-
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.
3.2.1.78 73588
-
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.
3.2.1.78 76309
-
1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Vibrio sp. B5BQ69
-
-
3.2.1.78 Vibrio sp. MA-138 B5BQ69
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 proteolytic modification sequence contains a signal peptide of 26 amino acids Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 glucomannan + H2O from konjac flour Vibrio sp. ?
-
?
3.2.1.78 glucomannan + H2O from konjac flour Vibrio sp. MA-138 ?
-
?
3.2.1.78 additional information enzyme has the ability to bind soluble beta-mannans Vibrio sp. ?
-
?
3.2.1.78 additional information enzyme hydrolyzes homopolysaccharides such as codium mannan, Porphyra mannan, coffee mannan, and coconut mannan, to form mainly mannobiose and mannotriose in addition to minor sugars, such as mannose, mannotetraose, and mannopentaose Vibrio sp. ?
-
?
3.2.1.78 additional information enzyme has the ability to bind soluble beta-mannans Vibrio sp. MA-138 ?
-
?
3.2.1.78 additional information enzyme hydrolyzes homopolysaccharides such as codium mannan, Porphyra mannan, coffee mannan, and coconut mannan, to form mainly mannobiose and mannotriose in addition to minor sugars, such as mannose, mannotetraose, and mannopentaose Vibrio sp. MA-138 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 monomer 1 * 76309, calculated including signal peptide, 1 * 73588, calculated, mature protein, 1 * 73000, SDS-PAGE Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 Man5C
-
Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
-
Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 45
-
stable up to, for at least 20 min Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 7
-
-
Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 6.5 10 more than 80% of original activity after 12 h incubation at 4°C Vibrio sp.