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Literature summary extracted from

  • Ma, L.; Zhang, H.; Liu, Q.; Chen, J.; Zhang, J.; Chen, G.Q.
    Production of two monomer structures containing medium-chain-length polyhydroxyalkanoates by beta-oxidation-impaired mutant of Pseudomonas putida KT2442 (2009), Biores. Technol., 100, 4891-4894.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.211 degradation when gene encoding 3-hydroxyacyl-CoA dehydrogenase is deleted, it is possible to produce medium-chain-length polyhydroxyalkanoates containing only two different monomer structures Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.211 into XbaI- and HindIII-digested pK18mobsacB, resulting in plasmid pKXZ01 Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.211 additional information when gene encoding 3-hydroxyacyl-CoA dehydrogenase is partially or completely deleted, the polyhydroxyalkanoate accumulated has only two different monomer structures dominated by a monomer of the same chain length as that of the fatty acids fed and another monomer two carbon atoms shorter. Disruption of the 3-hydroxyacyl-CoA dehydrogenase gene dramatically reduces conversion of S-3-OH-acyl-CoA to ketoacyl-CoA, resulting in reduced beta-oxidation flux, thus, leading to the accumulation of other intermediates including enoyl-CoA which are used by the bacteria to synthesize polyhydroxyalkanoate Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.211 Pseudomonas putida Q88L88
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1.1.1.211 Pseudomonas putida KT 2240 Q88L88
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Synonyms

EC Number Synonyms Comment Organism
1.1.1.211 3-hydroxyacyl-CoA dehydrogenase
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 Pseudomonas putida