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Literature summary extracted from

  • Dilovic, I.; Gliubich, F.; Malpeli, G.; Zanotti, G.; Matkovic-Calogovic, D.
    Crystal structure of bovine 3-hydroxyanthranilate 3,4-dioxygenase (2009), Biopolymers, 91, 1189-1195.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.11.6 first vapor diffusion with sitting drop method starting from protein concentration of 10 mg/ml in 32% (NH4)2SO4, 0.1 M sodium acetate, 10 mM 2-mercaptoethanol, pH 5, subsequently, crystals can be obtained by seeding starting from fragments of first crystallization experiments using 40% (NH4)2SO4, Tris-HCl, 40 mM MgCl2, 3% MPD, pH 8 as precipitant Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.6 33000
-
 SDS-PAGE, nondenaturating PAGE resolves two components, purified by FPLC on protein PakGlass DEAE-4 PW column, one component is N-terminally truncated annexin IV Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.6 2-amino-3-hydroxybenzoic acid + O2 Bos taurus
-
 2,3-pyridinedicarboxylic acid + H2O intermediate 2-amino-3-carboxymuconic acid semialdehyde ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.6 Bos taurus Q0VCA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.6 bovine kidney homogenized in 0.1 M potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, centrifuged, protein fraction of supernatant that precipitates from 34-62% saturated (NH4)2SO4 is resuspended in 5 mM potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, dialyzed against same buffer containing protease inhibitor PMSF (0.1 mM), applied to DEAE Sephadex A-50 column, fractions with enzyme activity pooled and concentrated by precipitation with 80%-saturated (NH4)2SO4, dialyzed against 10 mM Tris-HCl with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, applied to Blue-Sepharose CL-4B column, concentration of active fractions by ultrafiltration through YM-10 membrane Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.6 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.6 2-amino-3-hydroxybenzoic acid + O2
-
 Bos taurus 2,3-pyridinedicarboxylic acid + H2O intermediate 2-amino-3-carboxymuconic acid semialdehyde ?

Subunits

EC Number Subunits Comment Organism
1.13.11.6 monomer 1 * 33000, SDS-PAGE, 286 amino acids, 2 domains that represent the dimers of the prokaryote enzyme structure which is also conserved in simple eukaryotes Bos taurus

Synonyms

EC Number Synonyms Comment Organism
1.13.11.6 3-hydroxyanthranilate 3,4-dioxygenase
-
 Bos taurus
1.13.11.6 3HAO
-
 Bos taurus