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Literature summary extracted from

  • Martinez-Rodriguez, S.; Encinar, J.A.; Hurtado-Gomez, E.; Prieto, J.; Clemente-Jimenez, J.M.; Las Heras-Vazquez, F.J.; Rodriguez-Vico, F.; Neira, J.L.
    Metal-triggered changes in the stability and secondary structure of a tetrameric dihydropyrimidinase: a biophysical characterization (2009), Biophys. Chem., 139, 42-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.2
-
Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.2 in the absence and in the presence of zinc, hanging drop vapour diffusion method Sinorhizobium meliloti

General Stability

EC Number General Stability Organism
3.5.2.2 the presence of Zn2+ does not only increase the percentage of secondary structure, but also the thermal and chemical stability of Dhp Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.2 Zn2+ the presence of Zn2+ does not only increase the percentage of secondary structure, but also the thermal and chemical stability of SmelDhp Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.2 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.2
-
Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.2 5,6-dihydrothymine + H2O
-
Sinorhizobium meliloti N-carbamyl-beta-aminoisobutyrate
-
r
3.5.2.2 5,6-dihydrouracil + H2O
-
Sinorhizobium meliloti 3-ureidopropionate
-
r

Subunits

EC Number Subunits Comment Organism
3.5.2.2 tetramer in the absence of Zn2+, the protein acquires a tetrameric functional structure at pH 6.0, which is stable up to pH 9 Sinorhizobium meliloti

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.2 6 9 in the absence of Zn2+, the protein acquires a tetrameric functional structure at pH 6.0, which is stable up to pH 9 Sinorhizobium meliloti