Literature summary extracted from

Sovic, D.; Lester, L.; Murray, E.; Cohenford, M.
The utilization of bathocuproinedisulfonic acid as a reagent for determining D-glucose and D-galactose levels in glycoconjugates (2008), Bioorg. Chem., 36, 91-95.

Application

EC Number	Application	Comment	Organism
1.1.1.119	analysis	method for determination of D-glucose- and D-galactose levels in glycoconjugates. The NAD(P)H produced from the enzymatic oxidation of the monosaccharides reacts with a CuSO4-bathocuproinedisulfonic acid reagent to produce a color complex absorbing maximally at 486 nm. With galactose dehydrogenase and glucose dehydrogenase serving as the model enzymes, reaction analysis gives a linear plot from 2.5 to 250 nmol of sugar. Method has been applicated to sugar released by acid hydrolysis from lactose, porcine submaxillary mucin and raffinose was quantified	Pseudomonas fluorescens

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.119	Pseudomonas fluorescens	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.1.1.119	commercial preparation	-	Pseudomonas fluorescens	-

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Release 2023.1 (January 2023)