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Literature summary extracted from

  • Soyut, H.; Beydemir, S.; Hisar, O.
    Effects of some metals on carbonic anhydrase from brains of rainbow trout (2008), Biol. Trace Elem. Res., 123, 179-190.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 Ag+ silver inhibits competitively Oncorhynchus mykiss
4.2.1.1 Cd2+ cadmium inhibits competitively Oncorhynchus mykiss
4.2.1.1 Co2+ cobalt inhibits competitively Oncorhynchus mykiss
4.2.1.1 Cu2+ copper inhibits noncompetitively Oncorhynchus mykiss
4.2.1.1 Zn2+ zinc inhibits uncompetitively Oncorhynchus mykiss

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 27300
-
 native enzyme, estimated by gel filtration Oncorhynchus mykiss
4.2.1.1 29000
-
 subunit, SDS-PAGE Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 CO2 + H2O Oncorhynchus mykiss
-
 H2CO3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Oncorhynchus mykiss
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography Oncorhynchus mykiss

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 brain
-
 Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
 enzyme from lysate shows a specific activity of 1.77 EU/mg, the 1280fold purified enzyme shows a specific activity of 2275 EU/mg Oncorhynchus mykiss

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Oncorhynchus mykiss H2CO3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 carbonate hydrolyase
-
 Oncorhynchus mykiss
4.2.1.1 carbonic anhydrase
-
 Oncorhynchus mykiss

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 9
-
 in 1 M Tris-SO4 at 4°C Oncorhynchus mykiss

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.1 9
-
 stable at pH 9.0 in 1 M Tris-SO4 at 4°C Oncorhynchus mykiss

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.014
-
 Co2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
4.2.1.1 2.15
-
 Zn2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
4.2.1.1 27.68
-
 Cu2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
4.2.1.1 94.18
-
 Cd2+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss
4.2.1.1 193.9
-
 Ag+ in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.1 0.05
-
 in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Co2+
4.2.1.1 0.31
-
 in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Zn2+
4.2.1.1 30
-
 in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Cu2+
4.2.1.1 82.5
-
 in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Cd2+
4.2.1.1 159
-
 in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C Oncorhynchus mykiss Ag+