BRENDA - Enzyme Database

Kinetic characterization of recombinant mouse retinal dehydrogenase types 3 and 4 for retinal substrates

Sima, A.; Parisotto, M.; Mader, S.; Bhat, P.V.; Biochim. Biophys. Acta 1790, 1660-1664 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.2.1.36
expressed in Escherichia coli BL21(DE3) cells as His-tagged enzyme
Mus musculus
1.2.1.36
into the vector pET28a for expression in Escherichia coli BL21DE3 cells
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.36
beta-ionone
potent inhibitor of isoform RALDH4 activity, at 0.002 mM concentration 9-cis and 13-cis retinal oxidation is reduced by 53% and 63%, respectively
Mus musculus
1.2.1.36
Mg2+
4 mM Mg2+ inhibits 9-cis retinal oxidation by isoform RALDH4 by 30%
Mus musculus
1.2.1.36
additional information
beta-ionone has no effect on isoform RALDH3 activity
Mus musculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.36
0.003
-
9-cis retinal
-
Mus musculus
1.2.1.36
0.003
-
9-cis retinal
isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
1.2.1.36
0.0034
-
13-cis retinal
-
Mus musculus
1.2.1.36
0.0034
-
13-cis retinal
isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
1.2.1.36
0.0039
-
all-trans retinal
-
Mus musculus
1.2.1.36
0.0039
-
all-trans retinal
isoform RALDH3, in 10 mM Tris-HCl buffer, pH 8.5, at 37°C
Mus musculus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.36
cytosol
-
Mus musculus
5829
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.1.36
Mg2+
activity is slightly activated by magnesium at higher concentrations
Mus musculus
1.2.1.36
Mg2+
magnesium dramatically activated 13-cis retinal oxidation without significantly affecting 9-cis retinal oxidation
Mus musculus
1.2.1.36
Mg2+
Mg2+ activates 13-cis retinal oxidation by isoform RALDH4 by 3fold, does not significantly influence 9-cis retinal oxidation, and slightly activates isoform RALDH3 activity, owever, magnesium at lower concentration has no effect on RALDH4 activity
Mus musculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.36
55000
-
SDS-PAGE
Mus musculus
1.2.1.36
55000
-
determined by SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.36
retinal + NAD+ + H2O
Mus musculus
-
retinoate + NADH + H+
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.1.36
Mus musculus
-
-
-
1.2.1.36
Mus musculus
Q9JHW9
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.2.1.36
Ni-NTA column chromatography
Mus musculus
1.2.1.36
on a Ni-NTA column
Mus musculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.36
kidney
adult mouse
Mus musculus
-
1.2.1.36
liver
adult mouse
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.1.36
13-cis-retinal + NAD+ + H2O
-
696437
Mus musculus
13-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
13-cis-retinal + NAD+ + H2O
isoform RALDH4 oxidizes 13-cis retinal with lower catalytic efficiency than 9-cis retinal
696437
Mus musculus
13-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
9-cis-retinal + NAD+ + H2O
-
696437
Mus musculus
9-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
9-cis-retinal + NAD+ + H2O
isoform RALDH4 catalyzes 9-cis retinal oxidation with 3fold higher efficiency than 13-cis retinal
696437
Mus musculus
9-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
all-trans retinal + NAD+ + H2O
-
696437
Mus musculus
retinoate + NADH + H+
-
-
-
?
1.2.1.36
all-trans retinal + NAD+ + H2O
RALDH3 oxidizes all-trans retinal with high catalytic efficiency
696437
Mus musculus
all-trans-retinoate + NADH + H+
-
-
-
?
1.2.1.36
additional information
show no activity with either 9-cis or 13-cis retinal
696437
Mus musculus
?
-
-
-
?
1.2.1.36
additional information
isoform RALDH3 does not show activity for either 9-cis or 13-cis retinal substrates, isoform RALDH4 is inactive for all-trans retinal substrate
696437
Mus musculus
?
-
-
-
?
1.2.1.36
retinal + NAD+ + H2O
-
696437
Mus musculus
retinoate + NADH + H+
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.2.1.36
RALDH3
-
Mus musculus
1.2.1.36
RALDH3
isoform
Mus musculus
1.2.1.36
Raldh4
-
Mus musculus
1.2.1.36
Raldh4
isoform
Mus musculus
1.2.1.36
retinal dehydrogenase 3
-
Mus musculus
1.2.1.36
retinal dehydrogenase 4
-
Mus musculus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.36
37
-
activity assay
Mus musculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.36
8.5
-
activity assay
Mus musculus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.36
NAD+
-
Mus musculus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.36
0.00032
-
beta-ionone
isoform RALDH4, using 13-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
1.2.1.36
0.00061
-
beta-ionone
isoform RALDH4, using 9-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.36
expressed in Escherichia coli BL21(DE3) cells as His-tagged enzyme
Mus musculus
1.2.1.36
into the vector pET28a for expression in Escherichia coli BL21DE3 cells
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.36
NAD+
-
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.36
beta-ionone
-
Mus musculus
1.2.1.36
beta-ionone
potent inhibitor of isoform RALDH4 activity, at 0.002 mM concentration 9-cis and 13-cis retinal oxidation is reduced by 53% and 63%, respectively
Mus musculus
1.2.1.36
Mg2+
4 mM Mg2+ inhibits 9-cis retinal oxidation by isoform RALDH4 by 30%
Mus musculus
1.2.1.36
additional information
beta-ionone has no effect on isoform RALDH3 activity
Mus musculus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
1.2.1.36
0.00032
-
beta-ionone
isoform RALDH4, using 13-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
1.2.1.36
0.00061
-
beta-ionone
isoform RALDH4, using 9-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.2.1.36
0.003
-
9-cis retinal
-
Mus musculus
1.2.1.36
0.003
-
9-cis retinal
isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
1.2.1.36
0.0034
-
13-cis retinal
-
Mus musculus
1.2.1.36
0.0034
-
13-cis retinal
isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C
Mus musculus
1.2.1.36
0.0039
-
all-trans retinal
-
Mus musculus
1.2.1.36
0.0039
-
all-trans retinal
isoform RALDH3, in 10 mM Tris-HCl buffer, pH 8.5, at 37°C
Mus musculus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.36
cytosol
-
Mus musculus
5829
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.1.36
Mg2+
activity is slightly activated by magnesium at higher concentrations
Mus musculus
1.2.1.36
Mg2+
magnesium dramatically activated 13-cis retinal oxidation without significantly affecting 9-cis retinal oxidation
Mus musculus
1.2.1.36
Mg2+
Mg2+ activates 13-cis retinal oxidation by isoform RALDH4 by 3fold, does not significantly influence 9-cis retinal oxidation, and slightly activates isoform RALDH3 activity, owever, magnesium at lower concentration has no effect on RALDH4 activity
Mus musculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.36
55000
-
SDS-PAGE
Mus musculus
1.2.1.36
55000
-
determined by SDS-PAGE
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.36
retinal + NAD+ + H2O
Mus musculus
-
retinoate + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.36
Ni-NTA column chromatography
Mus musculus
1.2.1.36
on a Ni-NTA column
Mus musculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.36
kidney
adult mouse
Mus musculus
-
1.2.1.36
liver
adult mouse
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.1.36
13-cis-retinal + NAD+ + H2O
-
696437
Mus musculus
13-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
13-cis-retinal + NAD+ + H2O
isoform RALDH4 oxidizes 13-cis retinal with lower catalytic efficiency than 9-cis retinal
696437
Mus musculus
13-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
9-cis-retinal + NAD+ + H2O
-
696437
Mus musculus
9-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
9-cis-retinal + NAD+ + H2O
isoform RALDH4 catalyzes 9-cis retinal oxidation with 3fold higher efficiency than 13-cis retinal
696437
Mus musculus
9-cis-retinoate + NADH + H+
-
-
-
?
1.2.1.36
all-trans retinal + NAD+ + H2O
-
696437
Mus musculus
retinoate + NADH + H+
-
-
-
?
1.2.1.36
all-trans retinal + NAD+ + H2O
RALDH3 oxidizes all-trans retinal with high catalytic efficiency
696437
Mus musculus
all-trans-retinoate + NADH + H+
-
-
-
?
1.2.1.36
additional information
show no activity with either 9-cis or 13-cis retinal
696437
Mus musculus
?
-
-
-
?
1.2.1.36
additional information
isoform RALDH3 does not show activity for either 9-cis or 13-cis retinal substrates, isoform RALDH4 is inactive for all-trans retinal substrate
696437
Mus musculus
?
-
-
-
?
1.2.1.36
retinal + NAD+ + H2O
-
696437
Mus musculus
retinoate + NADH + H+
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.36
37
-
activity assay
Mus musculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.36
8.5
-
activity assay
Mus musculus