Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sima, A.; Parisotto, M.; Mader, S.; Bhat, P.V.
    Kinetic characterization of recombinant mouse retinal dehydrogenase types 3 and 4 for retinal substrates (2009), Biochim. Biophys. Acta, 1790, 1660-1664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36 expressed in Escherichia coli BL21(DE3) cells as His-tagged enzyme Mus musculus
1.2.1.36 into the vector pET28a for expression in Escherichia coli BL21DE3 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.36 beta-ionone potent inhibitor of isoform RALDH4 activity, at 0.002 mM concentration 9-cis and 13-cis retinal oxidation is reduced by 53% and 63%, respectively Mus musculus
1.2.1.36 Mg2+ 4 mM Mg2+ inhibits 9-cis retinal oxidation by isoform RALDH4 by 30% Mus musculus
1.2.1.36 additional information beta-ionone has no effect on isoform RALDH3 activity Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.003
-
 9-cis retinal
-
 Mus musculus
1.2.1.36 0.003
-
 9-cis retinal isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C Mus musculus
1.2.1.36 0.0034
-
 13-cis retinal
-
 Mus musculus
1.2.1.36 0.0034
-
 13-cis retinal isoform RALDH4, in 100 mM HEPES buffer, pH 8.5, at 37°C Mus musculus
1.2.1.36 0.0039
-
 all-trans retinal
-
 Mus musculus
1.2.1.36 0.0039
-
 all-trans retinal isoform RALDH3, in 10 mM Tris-HCl buffer, pH 8.5, at 37°C Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.36 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.36 Mg2+ activity is slightly activated by magnesium at higher concentrations Mus musculus
1.2.1.36 Mg2+ magnesium dramatically activated 13-cis retinal oxidation without significantly affecting 9-cis retinal oxidation Mus musculus
1.2.1.36 Mg2+ Mg2+ activates 13-cis retinal oxidation by isoform RALDH4 by 3fold, does not significantly influence 9-cis retinal oxidation, and slightly activates isoform RALDH3 activity, owever, magnesium at lower concentration has no effect on RALDH4 activity Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.36 55000
-
 SDS-PAGE Mus musculus
1.2.1.36 55000
-
 determined by SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.36 retinal + NAD+ + H2O Mus musculus
-
 retinoate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Mus musculus
-
-
-
1.2.1.36 Mus musculus Q9JHW9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36 Ni-NTA column chromatography Mus musculus
1.2.1.36 on a Ni-NTA column Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 kidney adult mouse Mus musculus
-
1.2.1.36 liver adult mouse Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 13-cis-retinal + NAD+ + H2O
-
 Mus musculus 13-cis-retinoate + NADH + H+
-
 ?
1.2.1.36 13-cis-retinal + NAD+ + H2O isoform RALDH4 oxidizes 13-cis retinal with lower catalytic efficiency than 9-cis retinal Mus musculus 13-cis-retinoate + NADH + H+
-
 ?
1.2.1.36 9-cis-retinal + NAD+ + H2O
-
 Mus musculus 9-cis-retinoate + NADH + H+
-
 ?
1.2.1.36 9-cis-retinal + NAD+ + H2O isoform RALDH4 catalyzes 9-cis retinal oxidation with 3fold higher efficiency than 13-cis retinal Mus musculus 9-cis-retinoate + NADH + H+
-
 ?
1.2.1.36 all-trans retinal + NAD+ + H2O
-
 Mus musculus retinoate + NADH + H+
-
 ?
1.2.1.36 all-trans retinal + NAD+ + H2O RALDH3 oxidizes all-trans retinal with high catalytic efficiency Mus musculus all-trans-retinoate + NADH + H+
-
 ?
1.2.1.36 additional information show no activity with either 9-cis or 13-cis retinal Mus musculus ?
-
 ?
1.2.1.36 additional information isoform RALDH3 does not show activity for either 9-cis or 13-cis retinal substrates, isoform RALDH4 is inactive for all-trans retinal substrate Mus musculus ?
-
 ?
1.2.1.36 retinal + NAD+ + H2O
-
 Mus musculus retinoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.36 RALDH3
-
 Mus musculus
1.2.1.36 RALDH3 isoform Mus musculus
1.2.1.36 Raldh4
-
 Mus musculus
1.2.1.36 Raldh4 isoform Mus musculus
1.2.1.36 retinal dehydrogenase 3
-
 Mus musculus
1.2.1.36 retinal dehydrogenase 4
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.36 37
-
 activity assay Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.36 8.5
-
 activity assay Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.36 NAD+
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.36 0.00032
-
 beta-ionone isoform RALDH4, using 13-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C Mus musculus
1.2.1.36 0.00061
-
 beta-ionone isoform RALDH4, using 9-cis retinal as substrate, in 100 mM HEPES buffer, pH 8.5, at 37°C Mus musculus