Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Quesada-Soriano, I.; Leal, I.; Casas-Solvas, J.M.; Vargas-Berenguel, A.; Baron, C.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.; Garcia-Fuentes, L.
    Kinetic and thermodynamic characterization of dUTP hydrolysis by Plasmodium falciparum dUTPase (2008), Biochim. Biophys. Acta, 1784, 1347-1355.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.1.23 drug development the enzyme is a potential drug target against malaria Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expression of the recombinant enzyme Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 2'-deoxyuridine competitive inhibition Plasmodium falciparum
3.6.1.23 alpha,beta-imido-dUTP competitive inhibition Plasmodium falciparum
3.6.1.23 dUDP competitive inhibition Plasmodium falciparum
3.6.1.23 dUMP product, competitive inhibition Plasmodium falciparum
3.6.1.23 dUTP
-
 Plasmodium falciparum
3.6.1.23 EDTA causes a 105fold decrease and a 12fold increase of the kcat and Km values for dUTP hydrolysis, respectively, compared to the dUTP-Mg2+ complex Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 additional information
-
 additional information Michaelis-Menten and ligand binding kinetics, overview. Thermodynamic parameters for the interaction between Plasmodium falciparum dUTPase and deoxyuridine derivatives at 25.2 °C and pH 7.0 Plasmodium falciparum
3.6.1.23 0.0019
-
 dUTP pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.0019
-
 dUTP-Mg2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.0027
-
 dUTP-Co2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.0137
-
 dUTP-Mn2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Co2+ can substitute the physiological cofactor Mg2+, however the kcat is significantly reduced compared to dUTP-Mg2+ Plasmodium falciparum
3.6.1.23 Mg2+ dependent on, the energetic barrier of the reaction is 4fold higher when Mg2+ is depleted Plasmodium falciparum
3.6.1.23 Mn2+ can substitute the physiological cofactor Mg2+, however the kcat is significantly reduced compared to dUTP-Mg2+ Plasmodium falciparum
3.6.1.23 NaCl maximal activity at 0.1 M Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Plasmodium falciparum
-
 dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant enzyme by gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Plasmodium falciparum dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O dUTPase catalyzes the hydrolysis of the alpha,beta-diphosphate bond of dUTP Plasmodium falciparum dUMP + diphosphate
-
 ?
3.6.1.23 dUTP-Co2+ + H2O
-
 Plasmodium falciparum dUMP-Co2+ + diphosphate
-
 ?
3.6.1.23 dUTP-Mg2+ + H2O
-
 Plasmodium falciparum dUMP-Mg2+ + diphosphate
-
 ?
3.6.1.23 dUTP-Mn2+ + H2O
-
 Plasmodium falciparum dUMP-Mn2+ + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 trimer
-
 Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Plasmodium falciparum
3.6.1.23 dUTPase
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.23 25
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 3.4
-
 dUTP pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 4.1
-
 dUTP-Co2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 5.3
-
 dUTP-Mn2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 13.3
-
 dUTP-Mg2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.23 7
-
 assay at Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.23 0.0718
-
 dUTP-Mn2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.092
-
 dUTP-Co2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.0955
-
 dUTP-Mg2+ pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.099
-
 dUMP pH 7.0, 25°C Plasmodium falciparum
3.6.1.23 0.115
-
 dUTP pH 7.0, 25°C, recombinant enzyme Plasmodium falciparum
3.6.1.23 0.121
-
 dUTP pH 7.0, 25°C, recombinant enzyme, without NaCl, presence of EDTA Plasmodium falciparum