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Literature summary extracted from

  • Perdomo-Morales, R.; Montero-Alejo, V.; Perera, E.; Pardo-Ruiz, Z.; Alonso-Jimenez, E.
    Hemocyanin-derived phenoloxidase activity in the spiny lobster Panulirus argus (Latreille, 1804) (2008), Biochim. Biophys. Acta, 1780, 652-658.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.1 catechol catechol produces substrate inhibition above 16 mM Panulirus argus
1.10.3.1 dopamine dopamine produces substrate inhibition above 2 mM Panulirus argus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.1 0.181
-
 dopamine SDS-activated hemocyanin Panulirus argus
1.10.3.1 2.565
-
 L-Dopa SDS-activated hemocyanin Panulirus argus
1.10.3.1 7.174
-
 catechol SDS-activated hemocyanin Panulirus argus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.1 77000
-
 1 * 77000 + 1 * 80000 + 1 * 89000 Panulirus argus
1.10.3.1 80000
-
 1 * 77000 + 1 * 80000 + 1 * 89000 Panulirus argus
1.10.3.1 89000
-
 1 * 77000 + 1 * 80000 + 1 * 89000 Panulirus argus
1.10.3.1 170000
-
 incubation of native hemocyanin with trypsin, produces a band of 170000 Da with intense phenoloxidase activity Panulirus argus
1.10.3.1 190000
-
 incubation of native hemocyanin with chymotrypsin, produces a band of 190000 Da with intense phenoloxidase activity Panulirus argus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.1 Panulirus argus
-
 spiny lobster
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.1 Sephacryl S-300 gel filtration Panulirus argus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.1 hemolymph
-
 Panulirus argus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.1 2 catechol + O2
-
 Panulirus argus 2 1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 2 dopamine + O2 dopamine is the most specific substrate Panulirus argus 2 4-(2-aminoethyl)-1,2-benzoquinone + 2 H2O
-
 ?
1.10.3.1 L-Dopa + O2
-
 Panulirus argus L-dopaquinone + H2O
-
 ?
1.10.3.1 additional information phenoloxidase activity is not observed when tyramine and tyrosine (monophenols) were used as substrates Panulirus argus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.1 trimer 1 * 77000 + 1 * 80000 + 1 * 89000 Panulirus argus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.1 hemocyanin behaves as a catecholoxidase and shows phenoloxidase activity in vitro Panulirus argus
1.10.3.1 phenoloxidase
-
 Panulirus argus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.1 5.5
-
 there are two peaks of optimal pH, at pH 5.5 and pH 7.0 Panulirus argus
1.10.3.1 7
-
 there are two peaks of optimal pH, at pH 5.5 and pH 7.0 Panulirus argus