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Literature summary extracted from

  • Clarke, T.; Kawai, F.; Park, S.Y.; Tame, J.; Dowson, C.; Roper, D.
    Mutational analysis of the substrate specificity of Escherichia coli penicillin binding protein 4 (PBP4) (2009), Biochemistry, 48, 2675-2683.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.16.4 into the vector pET21b Escherichia coli K-12

Protein Variants

EC Number Protein Variants Comment Organism
3.4.16.4 D155A mutant, constructed for the demonstration of the relative importance of residue Asp155 Escherichia coli K-12
3.4.16.4 F160A mutant, constructed for the demonstration of the relative importance of residue Phe160 Escherichia coli K-12
3.4.16.4 Q422A mutant, constructed for the demonstration of the relative importance of residue Gln422 Escherichia coli K-12
3.4.16.4 Q422S mutant, constructed for the demonstration of the relative importance of residue Gln422 Escherichia coli K-12
3.4.16.4 R361A mutant, constructed for the demonstration of the relative importance of residue Arg361 Escherichia coli K-12
3.4.16.4 R361H mutant, constructed for the demonstration of the relative importance of residue Arg361 Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.16.4 0.0178
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant R361H Escherichia coli K-12
3.4.16.4 0.0204
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine wild type enzyme Escherichia coli K-12
3.4.16.4 0.0344
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant F160A Escherichia coli K-12
3.4.16.4 0.116
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant Q422S Escherichia coli K-12
3.4.16.4 0.222
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant D155A Escherichia coli K-12
3.4.16.4 0.237
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant Q422A Escherichia coli K-12
3.4.16.4 0.401
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant R361A Escherichia coli K-12
3.4.16.4 0.675
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant Q422S Escherichia coli K-12
3.4.16.4 0.678
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant Q422A Escherichia coli K-12
3.4.16.4 0.682
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant wild type enzyme Escherichia coli K-12
3.4.16.4 0.712
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant R361H Escherichia coli K-12
3.4.16.4 0.744
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant R361A Escherichia coli K-12
3.4.16.4 0.913
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant F160A Escherichia coli K-12

Organism

EC Number Organism UniProt Comment Textmining
3.4.16.4 Escherichia coli K-12 P24228
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.16.4
-
Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.16.4 N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
-
Escherichia coli K-12 N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + D-alanine
-
?
3.4.16.4 N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine + H2O
-
Escherichia coli K-12 N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanine + D-alanine
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.16.4 DD-Carboxypeptidase
-
Escherichia coli K-12
3.4.16.4 LMM-PBP
-
Escherichia coli K-12
3.4.16.4 low molecular mass penicillin binding protein
-
Escherichia coli K-12
3.4.16.4 PBP 4
-
Escherichia coli K-12
3.4.16.4 penicillin binding protein 4
-
Escherichia coli K-12
3.4.16.4 serine-type-D-Ala-D-Ala carboxypeptidase
-
Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.16.4 37
-
activity assay Escherichia coli K-12

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.16.4 2.42
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant R361H Escherichia coli K-12
3.4.16.4 2.51
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant F160A Escherichia coli K-12
3.4.16.4 2.68
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant R361H Escherichia coli K-12
3.4.16.4 2.78
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant R361A Escherichia coli K-12
3.4.16.4 2.87
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant R361A Escherichia coli K-12
3.4.16.4 2.91
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant Q422A Escherichia coli K-12
3.4.16.4 2.97
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant Q422S Escherichia coli K-12
3.4.16.4 2.98
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant D155A Escherichia coli K-12
3.4.16.4 3.01
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine mutant F160A Escherichia coli K-12
3.4.16.4 3.02
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant Q422S Escherichia coli K-12
3.4.16.4 3.13
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine wild type enzyme Escherichia coli K-12
3.4.16.4 3.18
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine mutant Q422A Escherichia coli K-12
3.4.16.4 3.47
-
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine wild type enzyme Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.16.4 7.6
-
activity assay Escherichia coli K-12