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Literature summary extracted from

  • Liu, S.W.; Chen, C.S.; Chang, S.S.; Mong, K.K.; Lin, C.H.; Chang, C.W.; Tang, C.Y.; Li, Y.K.
    Identification of essential residues of human alpha-L-fucosidase and tests of its mechanism (2009), Biochemistry, 48, 110-120.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.51 Sodium azide 1M, enhanced enzyme activity, only in mutants Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 expression in Escherichia coli stream BL21(DE3) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.063
-
 2-chloro-4-nitrophenyl alpha-L-fucopyranoside mutant E289G Homo sapiens
3.2.1.51 0.11
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E289Q, 50 mM sodium phosphate buffer, pH 6.8, 37°C, loss of catalytic activity Homo sapiens
3.2.1.51 0.12
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E289G, 50 mM sodium phosphate buffer, pH 6.8, 37°C, loss of catalytic activity Homo sapiens
3.2.1.51 0.27
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D258G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.28
-
 p-nitrophenyl alpha-L-fucopyranoside 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.28
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D158G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.28
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D276G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.28
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E135G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.28
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E275G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.28
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E70G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.3
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E79G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 1.04
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D225G, 50 mM sodium phosphate buffer, pH 6.8, 37°C, loss of catalytic activity Homo sapiens
3.2.1.51 1.06
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D225N, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Dictyostelium discoideum P10901
-
-
3.2.1.51 Drosophila melanogaster Q9VTJ4
-
-
3.2.1.51 Halocynthia roretzi Q8TA71
-
-
3.2.1.51 Homo sapiens P04066
-
-
3.2.1.51 Pongo abelii Q5RFI5
-
-
3.2.1.51 Schistosoma japonicum Q86FH9
-
-
3.2.1.51 Thermotoga maritima Q9WYE2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51 33fold Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 55°C, LB medium, 4 h Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O pH 6.8, 37°C Homo sapiens 2-chloro-4-nitrophenol + L-fucose
-
 ?
3.2.1.51 2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O pH 6.8, 37°C Homo sapiens 2-fluoro-4-nitrophenol + L-fucose
-
 ?
3.2.1.51 2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O pH 6.8, 37°C Homo sapiens 2-methyl-4-nitrophenol + L-fucose
-
 ?
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O sodium acetate buffer 50 mM, pH 5.0, 37°C Homo sapiens 4-nitrophenol + L-fucose
-
 ?
3.2.1.51 m-nitrophenyl alpha-L-fucopyranoside + H2O pH 6.8, 37°C Homo sapiens m-nitrophenol + L-fucose
-
 ?
3.2.1.51 p-cyanophenyl alpha-L-fucopyranoside + H2O pH 6.8, 37°C Homo sapiens p-cyanophenol + L-fucose
-
 ?
3.2.1.51 phenyl alpha-L-fucopyranoside + H2O pH 6.8, 37°C Homo sapiens phenol + L-fucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 alpha-L-fucosidase
-
 Thermotoga maritima
3.2.1.51 alpha-L-fucosidase
-
 Homo sapiens
3.2.1.51 h-Fuc
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 55
-
-
 Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 55
-
 pH 7.0, 4 h Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 0.003
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D225G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.003
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D225N, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.039
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E289Q, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 0.048
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E289G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 12.5
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D258G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 16
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E135G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 16.3
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E79G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 16.7
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D276G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 16.9
-
 p-nitrophenyl alpha-L-fucopyranoside mutant D158G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 16.9
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E275G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 16.9
-
 p-nitrophenyl alpha-L-fucopyranoside mutant E70G, 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens
3.2.1.51 17.1
-
 p-nitrophenyl alpha-L-fucopyranoside 50 mM sodium phosphate buffer, pH 6.8, 37°C Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.51 4.8 5.2 recombinant enzyme Homo sapiens
3.2.1.51 6 6.5 enzyme in active form Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 2.5 8 4 h, above and below decrease in enzyme activity Homo sapiens
3.2.1.51 7
-
 55°C, 4 h Homo sapiens