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Literature summary extracted from

  • Le, K.H.D.; Boussac, A.; Frangioni, B.; Leger, C.; Lederer, F.
    Interdomain contacts in flavocytochrome b2, a mutational analysis (2009), Biochemistry, 48, 10803-10809.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 A67L site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 A67Q site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 D72A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 E63K site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 F39A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 K73A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 L65A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 additional information introduction of a number of heme domain side chain substitutions in and around the interface to probe their effect on flavin to heme and cytochrome b2 electron transfer, overview Saccharomyces cerevisiae
1.1.2.3 N69K site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 P44A site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 P64Q site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 P64R site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 V70M site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae
1.1.2.3 Y74F site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 additional information
-
additional information stopped-flow, pre-steady-state and steady-state kinetics measuring electron transfer in artificial systems, redox potentials, overview Saccharomyces cerevisiae
1.1.2.3 0.02
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A Saccharomyces cerevisiae
1.1.2.3 0.032
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q Saccharomyces cerevisiae
1.1.2.3 0.051
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A Saccharomyces cerevisiae
1.1.2.3 0.061
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R Saccharomyces cerevisiae
1.1.2.3 0.065
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
1.1.2.3 0.069
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q Saccharomyces cerevisiae
1.1.2.3 0.085
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L Saccharomyces cerevisiae
1.1.2.3 0.092
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K Saccharomyces cerevisiae
1.1.2.3 0.097
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A Saccharomyces cerevisiae
1.1.2.3 0.105
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M Saccharomyces cerevisiae
1.1.2.3 0.109
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A Saccharomyces cerevisiae
1.1.2.3 0.113
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
1.1.2.3 0.119
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K Saccharomyces cerevisiae
1.1.2.3 0.131
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.3 mitochondrion intermembrane space Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ heme cofactor Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 L-lactate + ferricytochrome c Saccharomyces cerevisiae flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source pyruvate + ferrocytochrome c + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae P00175
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + cytochrome c
-
Saccharomyces cerevisiae pyruvate + oxidized cytochrome c
-
?
1.1.2.3 L-lactate + ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
?
1.1.2.3 L-lactate + ferricytochrome c flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
?
1.1.2.3 additional information during the catalytic cycle, electrons are transferred one by one from the reduced flavin to heme b2 in the same subunit Saccharomyces cerevisiae ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.2.3 tetramer
-
Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 flavocytochrome b2
-
Saccharomyces cerevisiae
1.1.2.3 L-lactate cytochrome c oxidoreductase
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.3 30
-
assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.3 6
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q Saccharomyces cerevisiae
1.1.2.3 39
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
1.1.2.3 62
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
1.1.2.3 63
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A Saccharomyces cerevisiae
1.1.2.3 87
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A Saccharomyces cerevisiae
1.1.2.3 108
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L Saccharomyces cerevisiae
1.1.2.3 139
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K Saccharomyces cerevisiae
1.1.2.3 143
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R Saccharomyces cerevisiae
1.1.2.3 155
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme Saccharomyces cerevisiae
1.1.2.3 164
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A Saccharomyces cerevisiae
1.1.2.3 165
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A Saccharomyces cerevisiae
1.1.2.3 168
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q Saccharomyces cerevisiae
1.1.2.3 171
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M Saccharomyces cerevisiae
1.1.2.3 184
-
cytochrome c pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K Saccharomyces cerevisiae
1.1.2.3 185
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q Saccharomyces cerevisiae
1.1.2.3 190
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A Saccharomyces cerevisiae
1.1.2.3 196
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
1.1.2.3 207
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q Saccharomyces cerevisiae
1.1.2.3 208
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L Saccharomyces cerevisiae
1.1.2.3 209
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A Saccharomyces cerevisiae
1.1.2.3 211
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A Saccharomyces cerevisiae
1.1.2.3 212
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R Saccharomyces cerevisiae
1.1.2.3 214
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A Saccharomyces cerevisiae
1.1.2.3 214
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme Saccharomyces cerevisiae
1.1.2.3 218
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K Saccharomyces cerevisiae
1.1.2.3 218
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K Saccharomyces cerevisiae
1.1.2.3 223
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A Saccharomyces cerevisiae
1.1.2.3 225
-
FAD pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.3 7
-
assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 cytochrome c the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain Saccharomyces cerevisiae
1.1.2.3 FAD the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain Saccharomyces cerevisiae
1.1.2.3 heme the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.2.3 physiological function flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c and enables the latter to grow on lactate as the sole carbon source Saccharomyces cerevisiae