EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.2.3 | A67L | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | A67Q | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | D72A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | E63K | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | F39A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | K73A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | L65A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | additional information | introduction of a number of heme domain side chain substitutions in and around the interface to probe their effect on flavin to heme and cytochrome b2 electron transfer, overview | Saccharomyces cerevisiae |
1.1.2.3 | N69K | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | P44A | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | P64Q | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | P64R | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | V70M | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
1.1.2.3 | Y74F | site-directed mutagenesis, comparison of the mutant kinetics in electron transfer from flavin to heme to the wild-type kinetics | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.3 | additional information | - |
additional information | stopped-flow, pre-steady-state and steady-state kinetics measuring electron transfer in artificial systems, redox potentials, overview | Saccharomyces cerevisiae | |
1.1.2.3 | 0.02 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A | Saccharomyces cerevisiae | |
1.1.2.3 | 0.032 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q | Saccharomyces cerevisiae | |
1.1.2.3 | 0.051 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A | Saccharomyces cerevisiae | |
1.1.2.3 | 0.061 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R | Saccharomyces cerevisiae | |
1.1.2.3 | 0.065 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
1.1.2.3 | 0.069 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q | Saccharomyces cerevisiae | |
1.1.2.3 | 0.085 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L | Saccharomyces cerevisiae | |
1.1.2.3 | 0.092 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K | Saccharomyces cerevisiae | |
1.1.2.3 | 0.097 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A | Saccharomyces cerevisiae | |
1.1.2.3 | 0.105 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M | Saccharomyces cerevisiae | |
1.1.2.3 | 0.109 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A | Saccharomyces cerevisiae | |
1.1.2.3 | 0.113 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
1.1.2.3 | 0.119 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K | Saccharomyces cerevisiae | |
1.1.2.3 | 0.131 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme | Saccharomyces cerevisiae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.2.3 | mitochondrion | intermembrane space | Saccharomyces cerevisiae | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.3 | Fe2+ | heme cofactor | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.3 | L-lactate + ferricytochrome c | Saccharomyces cerevisiae | flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source | pyruvate + ferrocytochrome c + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.2.3 | Saccharomyces cerevisiae | P00175 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.2.3 | (S)-lactate + cytochrome c | - |
Saccharomyces cerevisiae | pyruvate + oxidized cytochrome c | - |
? | |
1.1.2.3 | L-lactate + ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c + H+ | - |
? | |
1.1.2.3 | L-lactate + ferricytochrome c | flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c in the mitochondrial intermembrane space in yeast and enables the latter to grow on lactate as the sole carbon source | Saccharomyces cerevisiae | pyruvate + ferrocytochrome c + H+ | - |
? | |
1.1.2.3 | additional information | during the catalytic cycle, electrons are transferred one by one from the reduced flavin to heme b2 in the same subunit | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.2.3 | tetramer | - |
Saccharomyces cerevisiae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.2.3 | flavocytochrome b2 | - |
Saccharomyces cerevisiae |
1.1.2.3 | L-lactate cytochrome c oxidoreductase | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.2.3 | 30 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.2.3 | 6 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q | Saccharomyces cerevisiae | |
1.1.2.3 | 39 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
1.1.2.3 | 62 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
1.1.2.3 | 63 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A | Saccharomyces cerevisiae | |
1.1.2.3 | 87 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A | Saccharomyces cerevisiae | |
1.1.2.3 | 108 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L | Saccharomyces cerevisiae | |
1.1.2.3 | 139 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K | Saccharomyces cerevisiae | |
1.1.2.3 | 143 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R | Saccharomyces cerevisiae | |
1.1.2.3 | 155 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme | Saccharomyces cerevisiae | |
1.1.2.3 | 164 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A | Saccharomyces cerevisiae | |
1.1.2.3 | 165 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A | Saccharomyces cerevisiae | |
1.1.2.3 | 168 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q | Saccharomyces cerevisiae | |
1.1.2.3 | 171 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M | Saccharomyces cerevisiae | |
1.1.2.3 | 184 | - |
cytochrome c | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K | Saccharomyces cerevisiae | |
1.1.2.3 | 185 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67Q | Saccharomyces cerevisiae | |
1.1.2.3 | 190 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant L65A | Saccharomyces cerevisiae | |
1.1.2.3 | 196 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
1.1.2.3 | 207 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64Q | Saccharomyces cerevisiae | |
1.1.2.3 | 208 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant A67L | Saccharomyces cerevisiae | |
1.1.2.3 | 209 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant F39A | Saccharomyces cerevisiae | |
1.1.2.3 | 211 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P44A | Saccharomyces cerevisiae | |
1.1.2.3 | 212 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant P64R | Saccharomyces cerevisiae | |
1.1.2.3 | 214 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant D72A | Saccharomyces cerevisiae | |
1.1.2.3 | 214 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, wild-type enzyme | Saccharomyces cerevisiae | |
1.1.2.3 | 218 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant E63K | Saccharomyces cerevisiae | |
1.1.2.3 | 218 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant N69K | Saccharomyces cerevisiae | |
1.1.2.3 | 223 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant K73A | Saccharomyces cerevisiae | |
1.1.2.3 | 225 | - |
FAD | pH 7.0, 30°C, flavin turnover in pre-steady-state flavin reduction, mutant V70M | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.2.3 | 7 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.2.3 | cytochrome c | the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain | Saccharomyces cerevisiae | |
1.1.2.3 | FAD | the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain | Saccharomyces cerevisiae | |
1.1.2.3 | heme | the flavoenzyme possesses flavocytochrome b2 subunits, that each consists of an N-terminal cytochrome domain and a C-terminal flavodehydrogenase, FDH, domain | Saccharomyces cerevisiae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.2.3 | physiological function | flavocytochrome b2, i.e. L-lactate cytochrome c oxidoreductase, catalyzes L-lactate oxidation at the expense of cytochrome c and enables the latter to grow on lactate as the sole carbon source | Saccharomyces cerevisiae |