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Literature summary extracted from

  • Grochowski, L.L.; Xu, H.; White, R.H.
    Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis (2008), Biochemistry, 47, 3033-3037.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.68 expressed in Escherichia coli Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.68 0.036
-
 (2S)-2-phospholactate pH 7.5, 70°C Methanocaldococcus jannaschii
2.7.7.68 0.056
-
 GTP pH 7.5, 70°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.68 Co2+ 1 mM, 16fold activation Methanocaldococcus jannaschii
2.7.7.68 Fe2+ 1 mM, 10fold activation Methanocaldococcus jannaschii
2.7.7.68 Mg2+ 1 mM, 23fold activation Methanocaldococcus jannaschii
2.7.7.68 Mn2+ 1 mM, 6fold activation Methanocaldococcus jannaschii
2.7.7.68 Ni2+ 1 mM, 8fold activation Methanocaldococcus jannaschii
2.7.7.68 Zn2+ 1 mM, 3fold activation Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.68 25000
-
 2 * 25000, recombinant enzyme, SDS-PAGE Methanocaldococcus jannaschii
2.7.7.68 50000
-
 recombinant enzyme, gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.68 (2S)-2-phospholactate + GTP Methanocaldococcus jannaschii third step of coenzyme F420 biosynthesis lactyl-2-diphospho-5'-guanosine + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.68 Methanocaldococcus jannaschii Q58297
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.68 recombinant enzyme Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.68 additional information
-
 due to the instability of the product lactyl-2-diphospho-5'-guanosine, the reaction is monitored indirectly through a coupled assay with 7,8-didemethyl-8-hydroxy-5-deazaflavin catalyzed by 2-phospho-L-lactate transferase (CofD, MJ1256) to form coenzyme F420-0 Methanocaldococcus jannaschii
2.7.7.68 1.3
-
 assay with gamma-thioGTP Methanocaldococcus jannaschii
2.7.7.68 2.3
-
 assay with ITP Methanocaldococcus jannaschii
2.7.7.68 3.3
-
 assay with dGTP Methanocaldococcus jannaschii
2.7.7.68 3.5
-
 assay with GTP Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.68 (2S)-2-phospholactate + dGTP specific activity is about 95% of the activity with GTP Methanocaldococcus jannaschii (2S)-lactyl-2-diphospho-5'-(2'-deoxy)guanosine + diphosphate
-
 ?
2.7.7.68 (2S)-2-phospholactate + gamma-thiolGTP specific activity is about 65% of the activity with GTP Methanocaldococcus jannaschii ?
-
 ?
2.7.7.68 (2S)-2-phospholactate + GTP
-
 Methanocaldococcus jannaschii lactyl-2-diphospho-5'-guanosine + diphosphate
-
 ?
2.7.7.68 (2S)-2-phospholactate + GTP third step of coenzyme F420 biosynthesis Methanocaldococcus jannaschii lactyl-2-diphospho-5'-guanosine + diphosphate
-
 ?
2.7.7.68 (2S)-2-phospholactate + ITP specific activity is about 35% of the activity with GTP Methanocaldococcus jannaschii lactyl-2-diphospho-5'-inosine + diphosphate + diphosphate
-
 ?
2.7.7.68 additional information weak activity with GDP and ATP Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.68 dimer 2 * 25000, recombinant enzyme, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.7.7.68 cofC
-
 Methanocaldococcus jannaschii
2.7.7.68 MJ0887
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.68 90
-
 10 min, completely stable Methanocaldococcus jannaschii
2.7.7.68 100
-
 10 min, 62% loss of activity Methanocaldococcus jannaschii