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Literature summary extracted from

  • Li, H.; Graupner, M.; Xu, H.; White, R.H.
    CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii (2003), Biochemistry, 42, 9771-9778.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.2.31 additional information no change of CofE activity is observed when the enzyme is assayed with the addition of 10 mM dithiothreitol to the reaction mixture Methanocaldococcus jannaschii
6.3.2.34 additional information no change of CofE activity is observed when the enzyme is assayed with the addition of 10 mM dithiothreitol to the reaction mixture Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.31 expressed in Escherichia coli Methanocaldococcus jannaschii
6.3.2.34 expressed in Escherichia coli Methanocaldococcus jannaschii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.31 beta,gamma-CH2-GTP 5 mM, 56% inhibition Methanocaldococcus jannaschii
6.3.2.31 Ca2+ 10 mM, only 26% F420-0 is converted to F420-1 Methanocaldococcus jannaschii
6.3.2.31 Cs2+ in the presence of either Rb+ or Cs+ at 0.2 M concentration, the only product of reaction is F420-1 Methanocaldococcus jannaschii
6.3.2.31 GDP 5 mM, 67% inhibition Methanocaldococcus jannaschii
6.3.2.31 additional information no significant inhibition when CofE is incubated with the following compound (10 mM): L-aspartate, Lglutamine, L-homocysteic acid, or DL-amino-4-phosphono-butyric acid Methanocaldococcus jannaschii
6.3.2.31 Rb+ in the presence of either Rb+ or Cs+ at 0.2 M concentration, the only product of reaction is F420-1 Methanocaldococcus jannaschii
6.3.2.31 Zn2+ 10 mM, only 26% F420-0 is converted to F420-1 Methanocaldococcus jannaschii
6.3.2.34 beta,gamma-CH2-GTP 5 mM, 56% inhibition Methanocaldococcus jannaschii
6.3.2.34 Ca2+ 10 mM Methanocaldococcus jannaschii
6.3.2.34 Cs2+ in the presence of either Rb+ or Cs+ at 0.2 M concentration no gamma-F420-2 is formed Methanocaldococcus jannaschii
6.3.2.34 GDP 5 mM, 67% inhibition Methanocaldococcus jannaschii
6.3.2.34 additional information no significant inhibition when CofE is incubated with the following compound (10 mM): L-aspartate, L-glutamine, L-homocysteic acid, or DL-amino-4-phosphono-butyric acid Methanocaldococcus jannaschii
6.3.2.34 Ni2+ in the presence of NiCl2 no gamma-F420-2 is formed Methanocaldococcus jannaschii
6.3.2.34 Rb+ in the presence of either Rb+ or Cs+ at 0.2 M concentration no gamma-F420-2 is formed Methanocaldococcus jannaschii
6.3.2.34 Zn2+ 10 mM Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.31 0.001
-
 coenzyme gamma-F420-0 pH 8.5, 50°C Methanocaldococcus jannaschii
6.3.2.34 0.00021
-
 coenzyme gamma-F420-1 pH 8.5, 50°C Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.31 Co2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2 Methanocaldococcus jannaschii
6.3.2.31 K+ CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, with maximum stimulation occurring at 0.2 M KCl, NH4+ stimulates activity to a lesser extent, extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective Methanocaldococcus jannaschii
6.3.2.31 Mg2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity Methanocaldococcus jannaschii
6.3.2.31 Mn2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity Methanocaldococcus jannaschii
6.3.2.31 NH4+ CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, NH4+ stimulates activity to a lesser extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective Methanocaldococcus jannaschii
6.3.2.34 Co2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2 Methanocaldococcus jannaschii
6.3.2.34 K+ CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, with maximum stimulation occurring at 0.2 M KCl, NH4+ stimulates activity to a lesser extent, extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective Methanocaldococcus jannaschii
6.3.2.34 Mg2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity Methanocaldococcus jannaschii
6.3.2.34 Mn2+ divalent cation requirement, maximum CofE activity is observed with the addition of 10 mM MnCl2. Reactions containing 10 mM either MgCl2 or CoCl2 produce 40% of the F420-2 of that is produced with MnCl2. The combination of 5 mM MgCl2 and 2-5 mM of MnCl2 supports the highest activity Methanocaldococcus jannaschii
6.3.2.34 NH4+ CofE absolutely requires a monovalent cation for activity, the greatest extent of activation is achieved by K+, NH4+ stimulates activity to a lesser extent, whereas Na+ and Li+ have no effect on CofE activity. A mixture of Mn2+, Mg2+, and K+ is the most effective Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.31 27150
-
 2 * 27150, calculated from sequence Methanocaldococcus jannaschii
6.3.2.31 52100
-
 gel filtration Methanocaldococcus jannaschii
6.3.2.34 27150
-
 2 * 27150, calculated from sequence Methanocaldococcus jannaschii
6.3.2.34 52100
-
 gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.31 L-glutamate + GTP + coenzyme F420-0 Methanocaldococcus jannaschii step in the biosynthesis of coenzyme F420 GDP + phosphate + coenzyme gamma-F420-1
-
 ?
6.3.2.34 L-glutamate + GTP + coenzyme gamma-F420-1 Methanocaldococcus jannaschii step in the biosynthesis of coenzyme F420 GDP + phosphate + coenzyme gamma-F420-2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.31 Methanocaldococcus jannaschii Q58178
-
-
6.3.2.34 Methanocaldococcus jannaschii Q58178
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.31 recombinant enzyme Methanocaldococcus jannaschii
6.3.2.34 recombinant enzyme Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.31 L-glutamate + ATP + coenzyme F420-0
-
 Methanocaldococcus jannaschii ADP + phosphate + coenzyme F420-1
-
 ?
6.3.2.31 L-glutamate + dGTP + coenzyme F420-0 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 Methanocaldococcus jannaschii dGDP + phosphate + coenzyme F420-1
-
 ?
6.3.2.31 L-glutamate + GTP + coenzyme F420-0 step in the biosynthesis of coenzyme F420 Methanocaldococcus jannaschii GDP + phosphate + coenzyme gamma-F420-1
-
 ?
6.3.2.31 L-glutamate + GTP + coenzyme F420-0 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 Methanocaldococcus jannaschii GDP + phosphate + coenzyme F420-1
-
 ?
6.3.2.31 L-glutamate + UTP + coenzyme F420-0 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue attached via its alpha-NH2 to F420-0 Methanocaldococcus jannaschii UDP + phosphate + coenzyme F420-1
-
 ?
6.3.2.31 additional information CofE incubated with 10 mM beta-glutamate, D-glutamate, gamma-glutamylglutamate, DL-2-amino-3-phosphonopropionic acid, 2-carboxyethylphosphonic acid, or L-R-aminoadipic acid produces no F420-1 Methanocaldococcus jannaschii ?
-
 ?
6.3.2.34 L-glutamate + dGTP + coenzyme F420-0 maximum activity for coenzyme F420-2 formation is 25% of the maximum activity with GTP. Coenzyme F420-1 is coenzyme F420 with one glutamic acid residue (attached via its alpha-NH2 to F420-0), the second glutamate in bound via a gamma ligation Methanocaldococcus jannaschii dGDP + phosphate + coenzyme F420-1
-
 ?
6.3.2.34 L-glutamate + dGTP + coenzyme gamma-F420-1 maximum activity for coenzyme F420-2 formation is 25% of the maximum activity with GTP. Coenzyme F420-1 is coenzyme F420 with one glutamic acid residue (attached via its alpha-NH2 to F420-0), the second glutamate in bound via a gamma ligation Methanocaldococcus jannaschii dGDP + phosphate + coenzyme gamma-F420-2
-
 ?
6.3.2.34 L-glutamate + GTP + coenzyme gamma-F420-1 step in the biosynthesis of coenzyme F420 Methanocaldococcus jannaschii GDP + phosphate + coenzyme gamma-F420-2
-
 ?
6.3.2.34 L-glutamate + GTP + coenzyme gamma-F420-1 coenzyme F420-1 is coenzyme F420 with one glutamic acid residue (attached via its alpha-NH2 to F420-0), the second glutamate in bound via a gamma ligation Methanocaldococcus jannaschii GDP + phosphate + coenzyme gamma-F420-2
-
 ?
6.3.2.34 L-glutamate + UTP + coenzyme gamma-F420-1 maximum activity for coenzyme F420-2 formation is 66% of the maximum activity with GTP. Coenzyme F420-1 is coenzyme F420 with one glutamic acid residue (attached via its alpha-NH2 to F420-0), the second glutamate in bound via a gamma ligation Methanocaldococcus jannaschii UDP + phosphate + coenzyme gamma-F420-2
-
 ?
6.3.2.34 additional information CofE incubated with 10 mM beta-glutamate, D-glutamate, gamma-glutamylglutamate, DL-2-amino-3-phosphonopropionic acid, 2-carboxyethylphosphonic acid, or L-R-aminoadipic acid produces no F420-2 or other F420 analogues. CofE cannot use F420-2 as substrate to add more glutamate residues Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.31 homodimer 2 * 27150, calculated from sequence Methanocaldococcus jannaschii
6.3.2.34 homodimer 2 * 27150, calculated from sequence Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
6.3.2.31 CofE
-
 Methanocaldococcus jannaschii
6.3.2.31 F420-0:gamma-glutamyl ligase
-
 Methanocaldococcus jannaschii
6.3.2.31 MJ0768
-
 Methanocaldococcus jannaschii
6.3.2.34 CofE
-
 Methanocaldococcus jannaschii
6.3.2.34 F420-0:gamma-glutamyl ligase
-
 Methanocaldococcus jannaschii
6.3.2.34 MJ0768
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.31 60
-
-
 Methanocaldococcus jannaschii
6.3.2.34 60
-
-
 Methanocaldococcus jannaschii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.2.31 35 80 35°C: about 30% of maximal activity, 80°C: about 55% of maximal activity Methanocaldococcus jannaschii
6.3.2.34 35 80 35°C: about 30% of maximal activity, 80°C: about 55% of maximal activity Methanocaldococcus jannaschii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.31 80
-
 15 min, 30% loss of activity Methanocaldococcus jannaschii
6.3.2.34 80
-
 15 min, 30% loss of activity Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.31 8.5
-
 with 50 mM CHES/Na+ buffer Methanocaldococcus jannaschii
6.3.2.34 8.5
-
 with 50 mM CHES/Na+ buffer Methanocaldococcus jannaschii