EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.75 | D153A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | D164A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | D42A | 20fold decrease in kcat | Escherichia coli |
2.5.1.75 | E173A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | E229A | substantial increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations | Escherichia coli |
2.5.1.75 | F84A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | H67F | 25fold increase in Km for dimethylallyl diphosphate | Escherichia coli |
2.5.1.75 | K23A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | K280A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | K56A | 15fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations | Escherichia coli |
2.5.1.75 | Q253A | 18fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations | Escherichia coli |
2.5.1.75 | Q282A | increase in both Km and kcat value | Escherichia coli |
2.5.1.75 | R167A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | R170A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | R213A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | R217A | increase in Km and decrease in kcat for dimethylallyl diphosphate | Escherichia coli |
2.5.1.75 | R281A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | S178A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | S43A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | T108A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | T19A | about 600fold decrease in kcat | Escherichia coli |
2.5.1.75 | T24A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | T275A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | T54A | increase in Km for both substrates | Escherichia coli |
2.5.1.75 | W285A | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | Y111F | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | Y111S | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | Y47F | at least 25% of wild-type activity | Escherichia coli |
2.5.1.75 | Y47S | 100fold decrease in kcat | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.75 | 1.4 | - |
dimethylallyl diphosphate | mutant Y47S, pH 8.0 | Escherichia coli | |
2.5.1.75 | 1.5 | - |
seventeen-base RNA oligonucleotide | mutant H67F, pH 8.0 | Escherichia coli | |
2.5.1.75 | 2.2 | - |
dimethylallyl diphosphate | wild-type, pH 8.0 | Escherichia coli | |
2.5.1.75 | 2.3 | - |
dimethylallyl diphosphate | mutant Q253A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 2.8 | - |
seventeen-base RNA oligonucleotide | mutant D42A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 2.9 | - |
dimethylallyl diphosphate | mutant D42A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 3.2 | - |
seventeen-base RNA oligonucleotide | wild-type, pH 8.0 | Escherichia coli | |
2.5.1.75 | 3.5 | - |
dimethylallyl diphosphate | mutant Y47F, pH 8.0 | Escherichia coli | |
2.5.1.75 | 4.4 | - |
seventeen-base RNA oligonucleotide | mutant Y47F, pH 8.0 | Escherichia coli | |
2.5.1.75 | 7.2 | - |
seventeen-base RNA oligonucleotide | mutant R217A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 8.9 | - |
seventeen-base RNA oligonucleotide | mutant Y47S, pH 8.0 | Escherichia coli | |
2.5.1.75 | 10 | - |
dimethylallyl diphosphate | mutant K56A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 12 | - |
seventeen-base RNA oligonucleotide | mutant T54A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 13 | - |
dimethylallyl diphosphate | mutant T54A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 16 | - |
seventeen-base RNA oligonucleotide | mutant T108A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 22 | - |
dimethylallyl diphosphate | mutant T108A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 27 | - |
dimethylallyl diphosphate | mutant d164A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 27 | - |
seventeen-base RNA oligonucleotide | mutant d164A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 45 | - |
seventeen-base RNA oligonucleotide | mutant Q282A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 50 | - |
dimethylallyl diphosphate | mutant H67F, pH 8.0 | Escherichia coli | |
2.5.1.75 | 50 | - |
seventeen-base RNA oligonucleotide | mutant K56A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 56 | - |
seventeen-base RNA oligonucleotide | mutant Q253A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 60 | - |
dimethylallyl diphosphate | mutant Q282A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 75 | - |
seventeen-base RNA oligonucleotide | mutant R167A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 114 | - |
dimethylallyl diphosphate | mutant R217A, pH 8.0 | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.75 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.75 | dimethylallyl diphosphate + seventeen-base RNA oligonucleotide | sequence 5'GCGGACUCAAAAUCCGC3', RNA oligonucleotide based on the unmodified stem-loop region of tRNAPhe | Escherichia coli | diphosphate + seventeen-base RNA oligonucleotide containing 6-dimethylallyladenosine | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.75 | 0.002 | - |
seventeen-base RNA oligonucleotide | mutant Y47S, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.007 | - |
seventeen-base RNA oligonucleotide | mutant R217A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.009 | - |
seventeen-base RNA oligonucleotide | mutant D42A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.07 | - |
seventeen-base RNA oligonucleotide | mutant T108A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.07 | - |
seventeen-base RNA oligonucleotide | mutant T54A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.15 | - |
seventeen-base RNA oligonucleotide | mutant D164A, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.18 | - |
seventeen-base RNA oligonucleotide | mutant H67F, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.19 | - |
seventeen-base RNA oligonucleotide | wild-type, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.23 | - |
seventeen-base RNA oligonucleotide | mutant Y47F, pH 8.0 | Escherichia coli | |
2.5.1.75 | 0.3 | - |
seventeen-base RNA oligonucleotide | mutnat Q282A, pH 8.0 | Escherichia coli |