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Literature summary extracted from

  • Soderberg, T.; Poulter, C.D.
    Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: site-directed mutagenesis of highly conserved residues (2001), Biochemistry, 40, 1734-1740.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.75 D153A at least 25% of wild-type activity Escherichia coli
2.5.1.75 D164A increase in Km for both substrates Escherichia coli
2.5.1.75 D42A 20fold decrease in kcat Escherichia coli
2.5.1.75 E173A at least 25% of wild-type activity Escherichia coli
2.5.1.75 E229A substantial increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations Escherichia coli
2.5.1.75 F84A at least 25% of wild-type activity Escherichia coli
2.5.1.75 H67F 25fold increase in Km for dimethylallyl diphosphate Escherichia coli
2.5.1.75 K23A increase in Km for both substrates Escherichia coli
2.5.1.75 K280A increase in Km for both substrates Escherichia coli
2.5.1.75 K56A 15fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations Escherichia coli
2.5.1.75 Q253A 18fold increase in Km for RNA, dimethylallyl diphosphate becomes inhibitory at higher concentrations Escherichia coli
2.5.1.75 Q282A increase in both Km and kcat value Escherichia coli
2.5.1.75 R167A increase in Km for both substrates Escherichia coli
2.5.1.75 R170A increase in Km for both substrates Escherichia coli
2.5.1.75 R213A at least 25% of wild-type activity Escherichia coli
2.5.1.75 R217A increase in Km and decrease in kcat for dimethylallyl diphosphate Escherichia coli
2.5.1.75 R281A at least 25% of wild-type activity Escherichia coli
2.5.1.75 S178A at least 25% of wild-type activity Escherichia coli
2.5.1.75 S43A at least 25% of wild-type activity Escherichia coli
2.5.1.75 T108A increase in Km for both substrates Escherichia coli
2.5.1.75 T19A about 600fold decrease in kcat Escherichia coli
2.5.1.75 T24A increase in Km for both substrates Escherichia coli
2.5.1.75 T275A at least 25% of wild-type activity Escherichia coli
2.5.1.75 T54A increase in Km for both substrates Escherichia coli
2.5.1.75 W285A at least 25% of wild-type activity Escherichia coli
2.5.1.75 Y111F at least 25% of wild-type activity Escherichia coli
2.5.1.75 Y111S at least 25% of wild-type activity Escherichia coli
2.5.1.75 Y47F at least 25% of wild-type activity Escherichia coli
2.5.1.75 Y47S 100fold decrease in kcat Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.75 1.4
-
dimethylallyl diphosphate mutant Y47S, pH 8.0 Escherichia coli
2.5.1.75 1.5
-
seventeen-base RNA oligonucleotide mutant H67F, pH 8.0 Escherichia coli
2.5.1.75 2.2
-
dimethylallyl diphosphate wild-type, pH 8.0 Escherichia coli
2.5.1.75 2.3
-
dimethylallyl diphosphate mutant Q253A, pH 8.0 Escherichia coli
2.5.1.75 2.8
-
seventeen-base RNA oligonucleotide mutant D42A, pH 8.0 Escherichia coli
2.5.1.75 2.9
-
dimethylallyl diphosphate mutant D42A, pH 8.0 Escherichia coli
2.5.1.75 3.2
-
seventeen-base RNA oligonucleotide wild-type, pH 8.0 Escherichia coli
2.5.1.75 3.5
-
dimethylallyl diphosphate mutant Y47F, pH 8.0 Escherichia coli
2.5.1.75 4.4
-
seventeen-base RNA oligonucleotide mutant Y47F, pH 8.0 Escherichia coli
2.5.1.75 7.2
-
seventeen-base RNA oligonucleotide mutant R217A, pH 8.0 Escherichia coli
2.5.1.75 8.9
-
seventeen-base RNA oligonucleotide mutant Y47S, pH 8.0 Escherichia coli
2.5.1.75 10
-
dimethylallyl diphosphate mutant K56A, pH 8.0 Escherichia coli
2.5.1.75 12
-
seventeen-base RNA oligonucleotide mutant T54A, pH 8.0 Escherichia coli
2.5.1.75 13
-
dimethylallyl diphosphate mutant T54A, pH 8.0 Escherichia coli
2.5.1.75 16
-
seventeen-base RNA oligonucleotide mutant T108A, pH 8.0 Escherichia coli
2.5.1.75 22
-
dimethylallyl diphosphate mutant T108A, pH 8.0 Escherichia coli
2.5.1.75 27
-
dimethylallyl diphosphate mutant d164A, pH 8.0 Escherichia coli
2.5.1.75 27
-
seventeen-base RNA oligonucleotide mutant d164A, pH 8.0 Escherichia coli
2.5.1.75 45
-
seventeen-base RNA oligonucleotide mutant Q282A, pH 8.0 Escherichia coli
2.5.1.75 50
-
dimethylallyl diphosphate mutant H67F, pH 8.0 Escherichia coli
2.5.1.75 50
-
seventeen-base RNA oligonucleotide mutant K56A, pH 8.0 Escherichia coli
2.5.1.75 56
-
seventeen-base RNA oligonucleotide mutant Q253A, pH 8.0 Escherichia coli
2.5.1.75 60
-
dimethylallyl diphosphate mutant Q282A, pH 8.0 Escherichia coli
2.5.1.75 75
-
seventeen-base RNA oligonucleotide mutant R167A, pH 8.0 Escherichia coli
2.5.1.75 114
-
dimethylallyl diphosphate mutant R217A, pH 8.0 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.75 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.75 dimethylallyl diphosphate + seventeen-base RNA oligonucleotide sequence 5'GCGGACUCAAAAUCCGC3', RNA oligonucleotide based on the unmodified stem-loop region of tRNAPhe Escherichia coli diphosphate + seventeen-base RNA oligonucleotide containing 6-dimethylallyladenosine
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.75 0.002
-
seventeen-base RNA oligonucleotide mutant Y47S, pH 8.0 Escherichia coli
2.5.1.75 0.007
-
seventeen-base RNA oligonucleotide mutant R217A, pH 8.0 Escherichia coli
2.5.1.75 0.009
-
seventeen-base RNA oligonucleotide mutant D42A, pH 8.0 Escherichia coli
2.5.1.75 0.07
-
seventeen-base RNA oligonucleotide mutant T108A, pH 8.0 Escherichia coli
2.5.1.75 0.07
-
seventeen-base RNA oligonucleotide mutant T54A, pH 8.0 Escherichia coli
2.5.1.75 0.15
-
seventeen-base RNA oligonucleotide mutant D164A, pH 8.0 Escherichia coli
2.5.1.75 0.18
-
seventeen-base RNA oligonucleotide mutant H67F, pH 8.0 Escherichia coli
2.5.1.75 0.19
-
seventeen-base RNA oligonucleotide wild-type, pH 8.0 Escherichia coli
2.5.1.75 0.23
-
seventeen-base RNA oligonucleotide mutant Y47F, pH 8.0 Escherichia coli
2.5.1.75 0.3
-
seventeen-base RNA oligonucleotide mutnat Q282A, pH 8.0 Escherichia coli