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Literature summary extracted from
- A novel variant of ferredoxin-dependent sulfite reductase having preferred substrate specificity for nitrite in the unicellular red alga Cyanidioschyzon merolae (2009), Biochem. J., 423, 91-98.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.7.1 | into the vector pTrc99A for expression in Escherichia coli cells | Cyanidioschyzon merolae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.7.1 | 0.0087 | - |
sulfite | - |
Cyanidioschyzon merolae |  |
| 1.8.7.1 | 0.221 | - |
nitrite | - |
Cyanidioschyzon merolae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.7.1 | chloroplast | - |
Cyanidioschyzon merolae | 9507 | - |
| 1.8.7.1 | plastid | - |
Cyanidioschyzon merolae | 9536 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.7.1 | hydrogen sulfide + oxidized ferredoxin + H2O | Cyanidioschyzon merolae | - |
sulfite + reduced ferredoxin + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.7.1 | Cyanidioschyzon merolae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.7.1 | cell extract is treated with DEAE-cellulose in a batchwise manner, the unbound fraction is fractionated by ammonium sulfate precipitation, anion-exchange and hydrophobic column chromatography are applied | Cyanidioschyzon merolae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.7.1 | leaf | - |
Cyanidioschyzon merolae | - |
| 1.8.7.1 | root | - |
Cyanidioschyzon merolae | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.7.1 | ammonium + oxidized ferredoxin + H2O | - |
Cyanidioschyzon merolae | nitrite + reduced ferredoxin + H+ | - |
? | |
| 1.8.7.1 | hydrogen sulfide + oxidized ferredoxin + H2O | - |
Cyanidioschyzon merolae | sulfite + reduced ferredoxin + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.7.1 | CMG021C | - |
Cyanidioschyzon merolae |
| 1.8.7.1 | CmSiRB | - |
Cyanidioschyzon merolae |
| 1.8.7.1 | ferredoxin-dependent sulfite reductase | - |
Cyanidioschyzon merolae |
| 1.8.7.1 | SIR | - |
Cyanidioschyzon merolae |
| 1.8.7.1 | sulfite reductase B | - |
Cyanidioschyzon merolae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.7.1 | 25 | - |
activity assay | Cyanidioschyzon merolae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.7.1 | 4.7 | - |
sulfite | - |
Cyanidioschyzon merolae | |
| 1.8.7.1 | 243 | - |
nitrite | - |
Cyanidioschyzon merolae | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.7.1 | 7.5 | - |
activity assay | Cyanidioschyzon merolae |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.7.1 | Ferredoxin | - |
Cyanidioschyzon merolae | |
| 1.8.7.1 | iron-sulfur centre | - |
Cyanidioschyzon merolae | |
| 1.8.7.1 | siroheme | - |
Cyanidioschyzon merolae | |
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Release 2023.1 (January 2023)
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