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Literature summary extracted from

  • Balme, A.; Brunt, C.E.; Pallister, R.L.; Chapman, S.K.; Reid, G.A.
    Isolation and characterization of the flavin-binding domain of flavocytochrome b2 expressed independently in Escherichia coli (1995), Biochem. J., 309, 601-605.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 expression of flavocytochrome b2 flavin-binding domain in Escherichia coli Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 additional information
-
 additional information steady-state kinetic parameters and 2H kinetic isotope effects for the isolated flavin domain and intact, wild-type flavocytoochrome b2 Saccharomyces cerevisiae
1.1.2.3 0.01
-
 ferricytochrome c pH 7.5, 25°C, wild-type enzyme Saccharomyces cerevisiae
1.1.2.3 0.023
-
 ferricytochrome c pH 7.5, 25°C, recombinantly expressed flavocytochrome b2 flavin-binding domain Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.3 47000
-
 x * 47000, recombinantly expressed flavocytochrome b2 flavin-binding domain, SDS-PAGE Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3 recombinantly expressed flavocytochrome b2 flavin-binding domain Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c kcat/KM is 24000 fold lower with the recombinantly expressed flavocytochrome b2 flavin-binding domain compared to wild-type enzyme Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.3 ? x * 47000, recombinantly expressed flavocytochrome b2 flavin-binding domain, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 flavocytochrome b2
-
 Saccharomyces cerevisiae
1.1.2.3 L-lactate:cytochrome c oxidoreductase
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.3 additional information
-
 additional information steady-state kinetic parameters and 2H kinetic isotope effects for the isolated flavin domain and intact, wild-type flavocytoochrome b2 Saccharomyces cerevisiae
1.1.2.3 0.02
-
 ferricytochrome c pH 7.5, 25°C, recombinantly expressed flavocytochrome b2 flavin-binding domain Saccharomyces cerevisiae
1.1.2.3 207
-
 ferricytochrome c pH 7.5, 25°C, wild-type enzyme Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 FMN the C-terminal domain of the enzyme contains FMN Saccharomyces cerevisiae
1.1.2.3 heme the N-terminal domain of the enzyme contains protohaem IX Saccharomyces cerevisiae