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Literature summary extracted from

  • Mowat, C.G.; Gazur, B.; Campbell, L.P.; Chapman, S.K.
    Flavin-containing heme enzymes (2010), Arch. Biochem. Biophys., 493, 37-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.17
-
 Escherichia coli
1.14.12.17
-
 Cupriavidus necator

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.12.17
-
 Escherichia coli
1.14.12.17
-
 Cupriavidus necator

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 H373Q the mutation results in a 3–4 orders of magnitude decrease in kcat and a slight increase in L-lactate Km Saccharomyces cerevisiae
1.1.2.3 L230A the mutant flavocytochrome b2 displays increased selectivity for (S)-2-hydroxyoctanoate over L-lactate by a factor of 40 (kcat/Km) Saccharomyces cerevisiae
1.1.2.3 L230A/A198G the double mutant enzyme has a 6fold greater catalytic efficiency with L-mandelate than with L-lactate Saccharomyces cerevisiae
1.1.2.3 Y254F the mutant enzyme has a Vmax value some 28fold lower than that of the wild type enzyme and a slightly raised L-lactate Km value Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.12.17 carbon monoxide competitive inhibition Cupriavidus necator
1.14.12.17 carbon monoxide competitive inhibition Escherichia coli
1.14.12.17 carbon monoxide competitive inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.17 0.06
-
 O2 apparent KM Saccharomyces cerevisiae
1.14.12.17 0.08
-
 O2 apparent KM Cupriavidus necator
1.14.12.17 0.09
-
 O2 apparent KM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.3 57500
-
 4 * 57500, X-ray crystallography Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 L-lactate + ferricytochrome c Saccharomyces cerevisiae
-
 pyruvate + ferrocytochrome c + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NAD(P)H Saccharomyces cerevisiae
-
 nitrate + NAD(P)+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NAD(P)H Escherichia coli
-
 nitrate + NAD(P)+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NAD(P)H Cupriavidus necator
-
 nitrate + NAD(P)+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae P00175
-
-
1.14.12.17 Cupriavidus necator P39662
-
-
1.14.12.17 Escherichia coli P24232
-
-
1.14.12.17 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.17
-
 Escherichia coli
1.14.12.17
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 L-lactate + ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + ferrocytochrome c + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NAD(P)H
-
 Saccharomyces cerevisiae nitrate + NAD(P)+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NAD(P)H
-
 Escherichia coli nitrate + NAD(P)+ + H+
-
 ?
1.14.12.17 nitric oxide + O2 + NAD(P)H
-
 Cupriavidus necator nitrate + NAD(P)+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.3 homotetramer 4 * 57500, X-ray crystallography Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.1.2.3 flavocytochrome b2
-
 Saccharomyces cerevisiae
1.14.12.17 flavohemoglobin
-
 Saccharomyces cerevisiae
1.14.12.17 flavohemoglobin
-
 Escherichia coli
1.14.12.17 flavohemoglobin
-
 Cupriavidus necator
1.14.12.17 nitric oxide dioxygenase
-
 Saccharomyces cerevisiae
1.14.12.17 nitric oxide dioxygenase
-
 Escherichia coli
1.14.12.17 nitric oxide dioxygenase
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 cytochrome c
-
 Saccharomyces cerevisiae
1.1.2.3 heme
-
 Saccharomyces cerevisiae
1.14.12.17 flavin
-
 Saccharomyces cerevisiae
1.14.12.17 flavin
-
 Escherichia coli
1.14.12.17 flavin
-
 Cupriavidus necator
1.14.12.17 heme
-
 Saccharomyces cerevisiae
1.14.12.17 heme
-
 Escherichia coli
1.14.12.17 heme
-
 Cupriavidus necator
1.14.12.17 NADH
-
 Saccharomyces cerevisiae
1.14.12.17 NADH
-
 Escherichia coli
1.14.12.17 NADH
-
 Cupriavidus necator
1.14.12.17 NADPH
-
 Saccharomyces cerevisiae
1.14.12.17 NADPH
-
 Escherichia coli
1.14.12.17 NADPH
-
 Cupriavidus necator

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.12.17 additional information
-
 carbon monoxide Ki value less than 1 microM Saccharomyces cerevisiae
1.14.12.17 additional information
-
 carbon monoxide Ki value less than 1 microM Escherichia coli
1.14.12.17 additional information
-
 carbon monoxide Ki value less than 1 microM Cupriavidus necator

Expression

EC Number Organism Comment Expression
1.1.2.3 Saccharomyces cerevisiae flavocytochrome b2 production is induced by the presence of oxygen and L-lactate up

General Information

EC Number General Information Comment Organism
1.1.2.3 metabolism the pyruvate produced as a result of L-lactate oxidation is utilised by the Krebs cycle, but flavocytochrome b2 also forms part of a short respiratory electron transport chain which results in one ATP molecule being produced for every L-lactate consumed Saccharomyces cerevisiae