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Literature summary extracted from

  • Tarasev, M.; Pullela, S.; Ballou, D.P.
    Distal end of 105-125 loop - a putative reductase binding domain of phthalate dioxygenase (2009), Arch. Biochem. Biophys., 487, 10-18.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.7 expressed in Escherichia coli 41 (DE3) Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.14.12.7 H120A putative participation in phthalate dioxygenase reductase binding Burkholderia cepacia
1.14.12.7 K117A putative participation in phthalate dioxygenase reductase binding Burkholderia cepacia
1.14.12.7 K117A/K119A/H120A/K121A/Y123A putative participation in phthalate dioxygenase reductase binding Burkholderia cepacia
1.14.12.7 K119A putative participation in phthalate dioxygenase reductase binding Burkholderia cepacia
1.14.12.7 K121A putative participation in phthalate dioxygenase reductase binding Burkholderia cepacia
1.14.12.7 W94A adjacent to the Rieske center Burkholderia cepacia
1.14.12.7 W94F adjacent to the Rieske center Burkholderia cepacia
1.14.12.7 W94Y adjacent to the Rieske center Burkholderia cepacia
1.14.12.7 Y123A putative participation in phthalate dioxygenase reductase binding Burkholderia cepacia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.7 Fe2+ Rieske 2Fe-2S center Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.7 Burkholderia cepacia
-
-
-
1.14.12.7 Burkholderia cepacia DB01
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.7
-
 Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.7 phthalate + NADH + H+ + O2
-
 Burkholderia cepacia cis-4,5-dihydro-4,5-dihydroxyphthalate + NAD+
-
 ?
1.14.12.7 phthalate + NADH + H+ + O2
-
 Burkholderia cepacia DB01 cis-4,5-dihydro-4,5-dihydroxyphthalate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.7 homohexamer
-
 Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.14.12.7 phthalate dioxygenase
-
 Burkholderia cepacia