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Literature summary extracted from

  • Johnson, N.A.; Liu, F.; Weeks, P.D.; Hentzen, A.E.; Kruse, H.P.; Parker, J.J.; Laursen, M.; Nissen, P.; Costa, C.J.; Gatto, C.
    A tomato ER-type Ca2+-ATPase, LCA1, has a low thapsigargin-sensitivity and can transport manganese (2009), Arch. Biochem. Biophys., 481, 157-168.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.2.2.10 expressed in a Saccharomyces cerevisiae triple mutant defective in both a Golgi and vacuolar Ca2+ pump, strain K616 and strain HI227 Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
7.2.2.10 D366A catalytically inactive Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.2.2.10 cyclopiazonic acid highly sensitive to the specific inhibitor cyclopiazonic acid Solanum lycopersicum
7.2.2.10 eosin potent inhibitor Solanum lycopersicum
7.2.2.10 thapsigargin only mildly sensitive to the ER-type pump inhibitor thapsigargin Solanum lycopersicum
7.2.2.10 vanadate potent inhibitor Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.2.2.10 Ca2+ dependent on Solanum lycopersicum
7.2.2.10 Mn2+ LCA1 may also act as a Mn2+ pump, consistent with a possible role in secretory pathway divalent cation homeostasis Solanum lycopersicum

Organism

EC Number Organism UniProt Comment Textmining
7.2.2.10 Solanum lycopersicum Q42883
-
-

Storage Stability

EC Number Storage Stability Organism
7.2.2.10 -80°C, the activity of LCA1 prepared from yeast cells stored at -80°C is dramatically reduced when compared to LCA1 prepared from freshly harvested cells Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.2.2.10 ATP + H2O + Ca2+/cis
-
 Solanum lycopersicum ADP + phosphate + Ca2+/trans
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.2.2.10 LCA1 ER-type Ca2+-ATPase Solanum lycopersicum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
7.2.2.10 0.00016
-
 in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C Solanum lycopersicum cyclopiazonic acid
7.2.2.10 0.000635
-
 recombinant enzyme expressed in Saccharomyces cerevisiae strain HI227, in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C Solanum lycopersicum thapsigargin
7.2.2.10 0.00095
-
 recombinant enzyme expressed in Saccharomyces cerevisiae strain K616, in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C Solanum lycopersicum thapsigargin
7.2.2.10 0.0036
-
 in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C Solanum lycopersicum eosin
7.2.2.10 0.0057
-
 in 1 mM EGTA, 130 mM NaCl, 3 mM MgCl2, at 37°C Solanum lycopersicum vanadate