Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, J.S.; Tiwari, M.K.; Moon, H.J.; Jeya, M.; Ramu, T.; Oh, D.K.; Kim, I.W.; Lee, J.K.
    Identification and characterization of a novel nitrilase from Pseudomonas fluorescens Pf-5 (2009), Appl. Microbiol. Biotechnol., 83, 273-283.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.5.7 expression in Escherichia coli Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.7 Co2+ 1 mM, 8% residual activity Pseudomonas fluorescens
3.5.5.7 Cu2+ 1 mM, no residual activity Pseudomonas fluorescens
3.5.5.7 Hg2+ 1 mM, 10% residual activity Pseudomonas fluorescens
3.5.5.7 N-ethylmaleimide 0.1 mM, 48% residual activity Pseudomonas fluorescens
3.5.5.7 pyridoxal 5'-phosphate 0.1 mM, 30% residual activity Pseudomonas fluorescens
3.5.5.7 Zn2+ 1 mM, no residual activity Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.5.7 2 3 Fumaronitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 17.9
-
 Succinonitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 23.3
-
 acrylonitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 23.8
-
 Benzonitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 24
-
 glutaronitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 25.3
-
 sebaconitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 43.9
-
 Propionitrile pH 7.0, 30°C Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.7 33000
-
 4 * 33000, SDS-PAGE Pseudomonas fluorescens
3.5.5.7 138000
-
 gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.7 Pseudomonas fluorescens Q4KCL8
-
-
3.5.5.7 Pseudomonas fluorescens Pf-5 Q4KCL8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.5.7 1.7
-
 substrate succinonitrile Pseudomonas fluorescens
3.5.5.7 1.9
-
 substrate fumaronitrile Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.7 acrylonitrile + H2O
-
 Pseudomonas fluorescens acrylic acid + NH3
-
 ?
3.5.5.7 acrylonitrile + H2O
-
 Pseudomonas fluorescens Pf-5 acrylic acid + NH3
-
 ?
3.5.5.7 benzonitrile + 2 H2O
-
 Pseudomonas fluorescens benzoate + NH3
-
 ?
3.5.5.7 fumaronitrile + H2O
-
 Pseudomonas fluorescens fumaric acid + NH3
-
 ?
3.5.5.7 fumaronitrile + H2O
-
 Pseudomonas fluorescens Pf-5 fumaric acid + NH3
-
 ?
3.5.5.7 glutaronitrile + H2O
-
 Pseudomonas fluorescens glutarate + NH3
-
 ?
3.5.5.7 additional information enzyme displays a high specificity towards dinitrile substrates Pseudomonas fluorescens ?
-
 ?
3.5.5.7 additional information enzyme displays a high specificity towards dinitrile substrates Pseudomonas fluorescens Pf-5 ?
-
 ?
3.5.5.7 propionitrile + H2O
-
 Pseudomonas fluorescens propionate + NH3
-
 ?
3.5.5.7 propionitrile + H2O
-
 Pseudomonas fluorescens Pf-5 propionate + NH3
-
 ?
3.5.5.7 sebaconitrile + H2O
-
 Pseudomonas fluorescens ? + NH3
-
 ?
3.5.5.7 succinonitrile + H2O
-
 Pseudomonas fluorescens succinate + NH3
-
 ?
3.5.5.7 succinonitrile + H2O
-
 Pseudomonas fluorescens Pf-5 succinate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.7 tetramer 4 * 33000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.5.5.7 NitPf5
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.7 45
-
-
 Pseudomonas fluorescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.7 16
-
 50% of initial activity after 80 h Pseudomonas fluorescens
3.5.5.7 30
-
 50% of initial activity after 30 h Pseudomonas fluorescens
3.5.5.7 45
-
 50% of initial activity after 300 min Pseudomonas fluorescens
3.5.5.7 55
-
 50% of initial activity after 180 min Pseudomonas fluorescens
3.5.5.7 65
-
 50% of initial activity after 45 min Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.5.7 0.17
-
 Propionitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 0.22
-
 Benzonitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 0.33
-
 acrylonitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 3.3
-
 sebaconitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 5
-
 glutaronitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 8.8
-
 Succinonitrile pH 7.0, 30°C Pseudomonas fluorescens
3.5.5.7 9.2
-
 Fumaronitrile pH 7.0, 30°C Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.7 7
-
-
 Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.5.7 Pseudomonas fluorescens isoelectric focusing and calculated
-
 5.4