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Literature summary extracted from

  • Luo, H.; Wang, Y.; Wang, H.; Yang, J.; Yang, Y.; Huang, H.; Yang, P.; Bai, Y.; Shi, P.; Fan, Y.; Yao, B.
    A novel highly acidic beta-mannanase from the acidophilic fungus Bispora sp. MEY-1: gene cloning and overexpression in Pichia pastoris (2009), Appl. Microbiol. Biotechnol., 82, 453-461.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Bispora sp.

General Stability

EC Number General Stability Organism
3.2.1.78 enzyme is strongly resistant to pepsin and trypsin digestion Bispora sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Hg2+ strong Bispora sp.
3.2.1.78 additional information enzymatic activity is not significantly affected by ions such as Ca2+, Cr3+, Co2+, Zn2+, Na+, K+, and Mg2+ Bispora sp.
3.2.1.78 Sodium dodecyl sulfate strong Bispora sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Ag+ stimulation Bispora sp.
3.2.1.78 Fe3+ stimulation Bispora sp.
3.2.1.78 Mn2+ stimulation Bispora sp.
3.2.1.78 Ni2+ stimulation Bispora sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 46800
-
 x * 46800, SDS-PAGE Bispora sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bispora sp. B5LXD7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 3373
-
 pH 1.5, 65°C Bispora sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 glucomannan + H2O from konjac flour Bispora sp. ? 70% of the activity with locust bean gum ?
3.2.1.78 guar gum + H2O
-
 Bispora sp. ? 14.8% of the activity with locust bean gum ?
3.2.1.78 locust bean gum + H2O
-
 Bispora sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 46800, SDS-PAGE Bispora sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 65
-
-
 Bispora sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 40 70 more than 65% of maximum activity within Bispora sp.
3.2.1.78 70
-
 20 min, 50% residual activity Bispora sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 11.5
-
-
 Bispora sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 1 5.5 more than 70% of maximum activity within Bispora sp.