Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, N.; Meng, K.; Wang, Y.; Shi, P.; Luo, H.; Bai, Y.; Yang, P.; Yao, B.
    Cloning, expression, and characterization of a new xylanase with broad temperature adaptability from Streptomyces sp. S9 (2008), Appl. Microbiol. Biotechnol., 80, 231-240.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 beta-mercaptoethanol 10 mM enhances the activity about 1.6fold Streptomyces sp. S9
3.2.1.8 additional information Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect Streptomyces sp. S9

Application

EC Number Application Comment Organism
3.2.1.8 industry favorable properties of XynAS9, such as high activity over a wide temperature range, good thermal and pH stability, and less complex hydrolysis products, make this xylanase promising for various applications in the animal feed and biofuel industries Streptomyces sp. S9

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 into pET-22b(+) vector and expressed in Escherichia coli BL21 (DE3) Streptomyces sp. S9

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ 1 mM strongly inhibits Streptomyces sp. S9
3.2.1.8 Co2+ 10 mM partialy inhibits Streptomyces sp. S9
3.2.1.8 Cu2+ 10 mM partialy inhibits Streptomyces sp. S9
3.2.1.8 Hg2+ 1 mM completely inhibits Streptomyces sp. S9
3.2.1.8 K+ 10 mM partialy inhibits Streptomyces sp. S9
3.2.1.8 Mn2+ 10 mM partialy inhibits Streptomyces sp. S9
3.2.1.8 additional information Na+, Ca2+, Ni2+, Mg2+, Fe3+, Zn2+, Li+, EDTA and sodium dodecyl sulfate have no effect Streptomyces sp. S9
3.2.1.8 Pb2+ 10 mM partialy inhibits Streptomyces sp. S9

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Cr3+ 10 mM enhances the activity about 1.3fold Streptomyces sp. S9

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 46000
-
 1 * 46000, SDS-PAGE Streptomyces sp. S9

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces sp. S9 B4XVN1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 to electrophoretic homogeneity by ammonium sulfate precipitation, ion exchange chromatography, and hydrophobic interaction chromatography, 7.1fold purified, with a final activity yield of 18.1% Streptomyces sp. S9

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 63.6
-
 culture supernatant Streptomyces sp. S9
3.2.1.8 453.7
-
 7.1fold purified enzyme Streptomyces sp. S9

Storage Stability

EC Number Storage Stability Organism
3.2.1.8 4°C, 0.2 M Na2HPO4, 0.1 M citric acid, pH 6.5 Streptomyces sp. S9

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O xylose and xylobiose are the predominant products Streptomyces sp. S9 D-xylose + xylobiose + ?
-
 ?
3.2.1.8 oat spelt xylan + H2O xylose and xylobiose are the predominant products Streptomyces sp. S9 D-xylose + xylobiose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 46000, SDS-PAGE Streptomyces sp. S9

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Streptomyces sp. S9
3.2.1.8 XynAS9
-
 Streptomyces sp. S9

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
-
 Streptomyces sp. S9

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50 80 retains more than 65% of the maximum activity Streptomyces sp. S9

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6.5
-
-
 Streptomyces sp. S9