EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.203 | expression in Escherichia coli | Pseudomonas syringae pv. tomato |
1.1.1.203 | Udh subcloned into vector pTrc99A to produce pT1053 and transformed into Escherichia coli BL21 Star (DE3) to construct an Escherichia coli strain carrying INO1, MIOX, and udh | Pseudomonas syringae pv. tomato |
1.13.99.1 | expression in Escherichia coli | Mus musculus |
5.5.1.4 | expression in Escherichia coli | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.203 | Pseudomonas syringae pv. tomato | Q888H1 | - |
- |
1.1.1.203 | Pseudomonas syringae pv. tomato | Q888H1 | pv. tomato DC3000 | - |
1.1.1.203 | Pseudomonas syringae pv. tomato DC3000 | Q888H1 | - |
- |
1.1.1.203 | Pseudomonas syringae pv. tomato DC3000 | Q888H1 | pv. tomato DC3000 | - |
1.13.99.1 | Mus musculus | Q9QXN5 | - |
- |
5.5.1.4 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.203 | 10 | - |
value about, culture condition 1-3 days, 30°C | Pseudomonas syringae pv. tomato |
1.13.99.1 | 0.005 | - |
culture condition: 1 day, 30°C | Mus musculus |
1.13.99.1 | 0.008 | - |
culture condition: 2 day, 30°C | Mus musculus |
1.13.99.1 | 0.01 | - |
culture condition: 3 day, 30°C | Mus musculus |
1.13.99.1 | 0.015 | - |
culture condition: 12 h, without supplementation of myo-inositol | Mus musculus |
1.13.99.1 | 0.028 | - |
culture condition: without supplementation of myo-inositol | Mus musculus |
1.13.99.1 | 0.042 | - |
culture condition: 12 h, supplementation with 60 mM myo-inositol, 1 mM Fe(NH4)2(SO4)2, 2 mM L-cysteine | Mus musculus |
1.13.99.1 | 0.076 | - |
culture condition: 12 h, supplementation with 60 mM myo-inositol | Mus musculus |
1.13.99.1 | 0.18 | - |
culture condition: 6 h, supplementation with 60 mM myo-inositol, 1 mM Fe(NH4)2(SO4)2, 2 mM L-cysteine | Mus musculus |
1.13.99.1 | 0.43 | - |
culture condition: 6 h, supplementation with 60 mM myo-inositol | Mus musculus |
5.5.1.4 | 0.05 | - |
value about, culture condition: 2 days, 30°C | Saccharomyces cerevisiae |
5.5.1.4 | 0.06 | - |
value about, culture condition: 3 days, 30°C | Saccharomyces cerevisiae |
5.5.1.4 | 0.1 | - |
value about, culture condition: 1 day, 30°C | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.203 | D-glucuronate + NAD+ + H2O | inclusion of a third enzyme, Udh, apart from myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice, facilitates conversion of D-glucuronate to D-glucaric acid. Up to 1 g/l of glucaric acid is produced with the three enzymes, whereas only 0.27 g/l of glucuronic acid is produced in the system harboring the two genes of INO1 and MIOX. Udh activity is highest, more than 2fold higher than Ino1 activity and 3fold higher than MIOX activity. The high activity of Udh may pull glucose flux through the glucaric acid pathway, leading to a relatively higher titer of glucaric acid | Pseudomonas syringae pv. tomato | D-glucaric acid + NADH + H+ | - |
? | |
1.1.1.203 | D-glucuronate + NAD+ + H2O | inclusion of a third enzyme, Udh, apart from myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice, facilitates conversion of D-glucuronate to D-glucaric acid. Up to 1 g/l of glucaric acid is produced with the three enzymes, whereas only 0.27 g/l of glucuronic acid is produced in the system harboring the two genes of INO1 and MIOX. Udh activity is highest, more than 2fold higher than Ino1 activity and 3fold higher than MIOX activity. The high activity of Udh may pull glucose flux through the glucaric acid pathway, leading to a relatively higher titer of glucaric acid | Pseudomonas syringae pv. tomato DC3000 | D-glucaric acid + NADH + H+ | - |
? | |
1.1.1.203 | D-glucuronic acid + NAD+ + H2O | assay at pH 8.0 | Pseudomonas syringae pv. tomato | D-glucaric acid + NADH + H+ | - |
? | |
1.1.1.203 | D-glucuronic acid + NAD+ + H2O | assay at pH 8.0 | Pseudomonas syringae pv. tomato DC3000 | D-glucaric acid + NADH + H+ | - |
? | |
1.13.99.1 | myo-inositol + O2 | preincubation without substrate for 10 min at 30°C to acitvate the enzyme, assay at pH 8.0, 30°C | Mus musculus | D-glucuronic acid + H2O | - |
? | |
5.5.1.4 | D-glucose 6-phosphate | assay at pH 7.5, 37°C | Saccharomyces cerevisiae | D-myo-inositol 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.203 | Udh | - |
Pseudomonas syringae pv. tomato |
1.1.1.203 | uronate dehydrogenase | - |
Pseudomonas syringae pv. tomato |
1.13.99.1 | MIOX | - |
Mus musculus |
1.13.99.1 | Myo-inositol oxygenase | - |
Mus musculus |
5.5.1.4 | INO1 | - |
Saccharomyces cerevisiae |
5.5.1.4 | Myo-inositol-1-phosphate synthase | - |
Saccharomyces cerevisiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.99.1 | 30 | - |
assay at | Mus musculus |
5.5.1.4 | 37 | - |
assay at | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.203 | 8 | - |
assay at | Pseudomonas syringae pv. tomato |
1.13.99.1 | 8 | - |
assay at | Mus musculus |
5.5.1.4 | 7.5 | - |
assay at | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.203 | NAD+ | - |
Pseudomonas syringae pv. tomato |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.99.1 | physiological function | biological production of glucaric acid | Mus musculus |