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Literature summary extracted from

  • Zhou, Y.; Meng, Q.; You, D.; Li, J.; Chen, S.; Ding, D.; Zhou, X.; Zhou, H.; Bai, L.; Deng, Z.
    Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008 (2008), Appl. Environ. Microbiol., 74, 7235-7242.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.2.20 additional information overexpression of FscTE under the strong constitutive ermE*p promoter in the wild-type strain, does not suppress FR-008/candicidin formation Streptomyces sp.

Application

EC Number Application Comment Organism
3.1.2.20 degradation TEII can maintain effective polyketide biosynthesis by selectively removing the nonelongatable residues bound to acyl carrier proteins Streptomyces sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.20 FscTE cloned into vector pET28a and expressed in Escherichia coli BL21(DE3)/pLysE Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.20 additional information deletion of FscTE reduces approximately 90% of the FR-008/candicidin production, while the production level is well restored when FscTE is added back to the mutant in trans. Successful complementation of the FscTE mutant with FscTE and tylO, whereas no complementation with nonribosomal peptide synthetase TEII tycF and srfAD Streptomyces sp.
3.1.2.20 S129A introduced into mutant ZYJ-4 (shows only 10% of the original FR-008/candicidin production) provides no contribution to the restoration of FR-008/candicidin titer Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.2.20 32
-
 propionyl-S-n-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.
3.1.2.20 33.4
-
 acetyl-S-N-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.20 33855
-
 1 * 33855, sequence analysis Streptomyces sp.
3.1.2.20 35000
-
 1 * 35000, SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.20 Streptomyces sp.
-
-
-
3.1.2.20 Streptomyces sp. FR-008
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.20 to homogeneity by Ni2+ affinity column chromatography Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.20 acetyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. N-acetylcysteamine + acetate
-
 ?
3.1.2.20 acetyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. FR-008 N-acetylcysteamine + acetate
-
 ?
3.1.2.20 malonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. N-acetylcysteamine + malonate
-
 ?
3.1.2.20 malonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. FR-008 N-acetylcysteamine + malonate
-
 ?
3.1.2.20 methylmalonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. N-acetylcysteamine + methylmalonate
-
 ?
3.1.2.20 methylmalonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. FR-008 N-acetylcysteamine + methylmalonate
-
 ?
3.1.2.20 additional information p-aminobenzoyl-S-N-acetylcysteamine is no proper substrate Streptomyces sp. ?
-
 ?
3.1.2.20 additional information p-aminobenzoyl-S-N-acetylcysteamine is no proper substrate Streptomyces sp. FR-008 ?
-
 ?
3.1.2.20 propionyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. N-acetylcysteamine + propionate
-
 ?
3.1.2.20 propionyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. FR-008 N-acetylcysteamine + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.20 monomer 1 * 35000, SDS-PAGE Streptomyces sp.
3.1.2.20 monomer 1 * 33855, sequence analysis Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.2.20 FscTE
-
 Streptomyces sp.
3.1.2.20 TEII
-
 Streptomyces sp.
3.1.2.20 TEII associated with the FR-008/candicidin gene cluster
-
 Streptomyces sp.
3.1.2.20 type II thioesterase
-
 Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.2.20 1.193
-
 acetyl-S-N-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.
3.1.2.20 1.82
-
 propionyl-S-n-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.