EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.307 | bovine serum albumin | 1 mg/ml, 30% activation | Neurospora crassa | |
1.1.1.307 | EDTA | 1 mM, 5% activation | Neurospora crassa |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.307 | synthesis | this enzyme is one of the most active xylose reductases and may be used for the in vitro production of xylitol | Neurospora crassa |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.307 | expression in Escherichia coli as a His6-tagged fusion protein in high yield | Neurospora crassa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.307 | additional information | no effect 0.1% w/v sodium azide | Neurospora crassa | |
1.1.1.307 | NaCl | 50 mM, 25% inhibition | Neurospora crassa | |
1.1.1.307 | sodium phosphite | 200 mM, 37% inhibition | Neurospora crassa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.307 | 0.0018 | - |
NADPH | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 0.016 | - |
NADH | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 34 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADPH | Neurospora crassa | |
1.1.1.307 | 37 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADH | Neurospora crassa | |
1.1.1.307 | 40 | - |
L-arabinose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 70 | - |
D-ribose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 180 | - |
D-galactose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 360 | - |
D-glucose | pH 6.3, 25°C | Neurospora crassa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.307 | additional information | no effect: 1 mM CuSO4 | Neurospora crassa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.307 | 38400 | - |
2 * 38400, SDS-PAGE | Neurospora crassa |
1.1.1.307 | 53000 | - |
gel filtration | Neurospora crassa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.307 | Neurospora crassa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.307 | - |
Neurospora crassa |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.307 | 4°C, stable for several months | Neurospora crassa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.307 | D-galactose + NADPH + H+ | catalytic efficiency is 9.1% of the catalytic efficiency for D-xylose | Neurospora crassa | ? | - |
? | |
1.1.1.307 | D-glucose + NADPH + H+ | catalytic efficiency is 3.3% of the catalytic efficiency for D-xylose | Neurospora crassa | ? | - |
? | |
1.1.1.307 | D-ribose + NADPH + H+ | catalytic efficiency is 41% of the catalytic efficiency for D-xylose | Neurospora crassa | ? | - |
? | |
1.1.1.307 | D-xylose + NADH + H+ | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa | xylitol + NAD+ | - |
? | |
1.1.1.307 | D-xylose + NADPH + H+ | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa | xylitol + NADP+ | - |
? | |
1.1.1.307 | L-arabinose + NADPH + H+ | catalytic efficiency is 41% of the catalytic efficiency for D-xylose | Neurospora crassa | L-arabinitol + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.307 | dimer | 2 * 38400, SDS-PAGE | Neurospora crassa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.307 | 45 | 55 | - |
Neurospora crassa |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.307 | 25 | 65 | 25°C: about 55% of maximal activity, 65°C: about 65% of maximal activity | Neurospora crassa |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.307 | 21 | - |
at room temperature stable for more than 1 month | Neurospora crassa |
1.1.1.307 | 40 | - |
half-life: 94 min | Neurospora crassa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.307 | 310 | - |
NADH | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 310 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADH | Neurospora crassa | |
1.1.1.307 | 1320 | - |
D-glucose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 1800 | - |
D-galactose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 1800 | - |
L-arabinose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 3120 | - |
D-ribose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 3600 | - |
NADPH | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 3600 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADPH | Neurospora crassa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.307 | 5.5 | - |
- |
Neurospora crassa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.307 | 4 | 7 | pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity | Neurospora crassa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.307 | NADH | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa | |
1.1.1.307 | NADPH | catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH | Neurospora crassa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.307 | 0.05 | - |
D-glucose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 0.16 | - |
D-galactose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 0.75 | - |
L-arabinose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 0.75 | - |
D-ribose | pH 6.3, 25°C | Neurospora crassa | |
1.1.1.307 | 1.8 | - |
D-xylose | pH 6.3, 25°C, cosubstrate: NADPH | Neurospora crassa | |
1.1.1.307 | 33.3 | - |
NADPH | pH 6.3, 25°C | Neurospora crassa |