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Literature summary extracted from

  • Woodyer, R.; Simurdiak, M.; van der Donk, W.A.; Zhao, H.
    Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa (2005), Appl. Environ. Microbiol., 71, 1642-1647.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.307 bovine serum albumin 1 mg/ml, 30% activation Neurospora crassa
1.1.1.307 EDTA 1 mM, 5% activation Neurospora crassa

Application

EC Number Application Comment Organism
1.1.1.307 synthesis this enzyme is one of the most active xylose reductases and may be used for the in vitro production of xylitol Neurospora crassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.307 expression in Escherichia coli as a His6-tagged fusion protein in high yield Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.307 additional information no effect 0.1% w/v sodium azide Neurospora crassa
1.1.1.307 NaCl 50 mM, 25% inhibition Neurospora crassa
1.1.1.307 sodium phosphite 200 mM, 37% inhibition Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.307 0.0018
-
NADPH pH 6.3, 25°C Neurospora crassa
1.1.1.307 0.016
-
NADH pH 6.3, 25°C Neurospora crassa
1.1.1.307 34
-
D-xylose pH 6.3, 25°C, cosubstrate: NADPH Neurospora crassa
1.1.1.307 37
-
D-xylose pH 6.3, 25°C, cosubstrate: NADH Neurospora crassa
1.1.1.307 40
-
L-arabinose pH 6.3, 25°C Neurospora crassa
1.1.1.307 70
-
D-ribose pH 6.3, 25°C Neurospora crassa
1.1.1.307 180
-
D-galactose pH 6.3, 25°C Neurospora crassa
1.1.1.307 360
-
D-glucose pH 6.3, 25°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.307 additional information no effect: 1 mM CuSO4 Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.307 38400
-
2 * 38400, SDS-PAGE Neurospora crassa
1.1.1.307 53000
-
gel filtration Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.307 Neurospora crassa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.307
-
Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.1.1.307 4°C, stable for several months Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.307 D-galactose + NADPH + H+ catalytic efficiency is 9.1% of the catalytic efficiency for D-xylose Neurospora crassa ?
-
?
1.1.1.307 D-glucose + NADPH + H+ catalytic efficiency is 3.3% of the catalytic efficiency for D-xylose Neurospora crassa ?
-
?
1.1.1.307 D-ribose + NADPH + H+ catalytic efficiency is 41% of the catalytic efficiency for D-xylose Neurospora crassa ?
-
?
1.1.1.307 D-xylose + NADH + H+ catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa xylitol + NAD+
-
?
1.1.1.307 D-xylose + NADPH + H+ catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa xylitol + NADP+
-
?
1.1.1.307 L-arabinose + NADPH + H+ catalytic efficiency is 41% of the catalytic efficiency for D-xylose Neurospora crassa L-arabinitol + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.307 dimer 2 * 38400, SDS-PAGE Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.307 45 55
-
Neurospora crassa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.307 25 65 25°C: about 55% of maximal activity, 65°C: about 65% of maximal activity Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.307 21
-
at room temperature stable for more than 1 month Neurospora crassa
1.1.1.307 40
-
half-life: 94 min Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.307 310
-
NADH pH 6.3, 25°C Neurospora crassa
1.1.1.307 310
-
D-xylose pH 6.3, 25°C, cosubstrate: NADH Neurospora crassa
1.1.1.307 1320
-
D-glucose pH 6.3, 25°C Neurospora crassa
1.1.1.307 1800
-
D-galactose pH 6.3, 25°C Neurospora crassa
1.1.1.307 1800
-
L-arabinose pH 6.3, 25°C Neurospora crassa
1.1.1.307 3120
-
D-ribose pH 6.3, 25°C Neurospora crassa
1.1.1.307 3600
-
NADPH pH 6.3, 25°C Neurospora crassa
1.1.1.307 3600
-
D-xylose pH 6.3, 25°C, cosubstrate: NADPH Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.307 5.5
-
-
Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.307 4 7 pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.307 NADH catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa
1.1.1.307 NADPH catalytic efficiency for NADPH is more than 100fold higher than the catalytic efficiency for NADH Neurospora crassa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.307 0.05
-
D-glucose pH 6.3, 25°C Neurospora crassa
1.1.1.307 0.16
-
D-galactose pH 6.3, 25°C Neurospora crassa
1.1.1.307 0.75
-
L-arabinose pH 6.3, 25°C Neurospora crassa
1.1.1.307 0.75
-
D-ribose pH 6.3, 25°C Neurospora crassa
1.1.1.307 1.8
-
D-xylose pH 6.3, 25°C, cosubstrate: NADPH Neurospora crassa
1.1.1.307 33.3
-
NADPH pH 6.3, 25°C Neurospora crassa