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Literature summary extracted from

  • Fahmy, A.S.; El-Beih, F.M.; Mohamed, S.A.; Abdel-Gany, S.S.; Abd-Elbaky, E.A.
    Characterization of an exopolygalacturonase from Aspergillus niger (2008), Appl. Biochem. Biotechnol., 149, 205-217.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.67 food industry properties of PGI may be suitable for food processing Aspergillus niger
3.2.1.82 food industry properties of PGI may be suitable for food processing Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ca2+ at 1 mM 21% inhibition, at 5 mM 35% inhibition Aspergillus niger
3.2.1.67 Co2+ at 1 mM 19% inhibition, at 5 mM 45% inhibition Aspergillus niger
3.2.1.67 Cu2+ at 1 mM 27% inhibition, at 5 mM 48% inhibition Aspergillus niger
3.2.1.67 Hg2+ at 1 mM 17% inhibition, at 5 mM 48% inhibition Aspergillus niger
3.2.1.67 Mg2+ at 1 mM 8% inhibition, at 5 mM 22% inhibition Aspergillus niger
3.2.1.67 Mn2+ at 1 mM 57% inhibition, at 5 mM 100% inhibition Aspergillus niger
3.2.1.67 additional information activity on pectin decreases with an increase in degree of esterification Aspergillus niger
3.2.1.67 Ni2+ at 1 mM 10% inhibition, at 5 mM 37% inhibition Aspergillus niger
3.2.1.82 CaCl2 at 1 mM causes 21% inhibition of PGI, at 5 mM causes 35% inhibition of PGI Aspergillus niger
3.2.1.82 CoCl2 at 1 mM causes 19% inhibition of PGI, at 5 mM causes 45% inhibition of PGI Aspergillus niger
3.2.1.82 CuSO4 at 1 mM causes 27% inhibition of PGI, at 5 mM causes 48% inhibition of PGI Aspergillus niger
3.2.1.82 HgCl2 at 1 mM causes 17% inhibition of PGI, at 5 mM causes 48% inhibition of PGI Aspergillus niger
3.2.1.82 MgCl2 at 1 mM causes 8% inhibition of PGI, at 5 mM causes 22% inhibition of PGI Aspergillus niger
3.2.1.82 MnCl2 at 1 mM causes 57% inhibition of PGI, at 5 mM causes complete inhibition of PGI Aspergillus niger
3.2.1.82 NiCl2 at 1 mM causes 10% inhibition of PGI, at 5 mM causes 37% inhibition of PGI Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 32000
-
 gel filtration Aspergillus niger
3.2.1.82 32000
-
 gel filtration of PGI Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Aspergillus niger
-
-
-
3.2.1.67 Aspergillus niger ATCC 9029
-
-
-
3.2.1.82 Aspergillus niger
-
-
-
3.2.1.82 Aspergillus niger ATCC 9029
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 12fold, by anion exchange chromatography and gel filtration, to homogeneity Aspergillus niger
3.2.1.82 PGI and PGII purified by gel filtration, PGI 12fold purified to homogeneity Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 34.7
-
 cell-free broth Aspergillus niger
3.2.1.67 418.6
-
 12fold purified enzyme Aspergillus niger
3.2.1.82 34.7
-
 cell-free broth of PGI Aspergillus niger
3.2.1.82 186.6
-
 PGI and PGII Aspergillus niger
3.2.1.82 418.6
-
 12fold purified PGI Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
3.2.1.67 4°C, 9 months Aspergillus niger
3.2.1.82 4°C, PGI in liquid form, 2 weeks Aspergillus niger
3.2.1.82 4°C, PGI in powder form, 9 months Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 pectin + H2O
-
 Aspergillus niger digalacturonate + D-galacturonic acid + ?
-
 ?
3.2.1.67 pectin + H2O
-
 Aspergillus niger ATCC 9029 digalacturonate + D-galacturonic acid + ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Aspergillus niger ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Aspergillus niger ATCC 9029 ?
-
 ?
3.2.1.82 pectin + H2O PGI activity on pectin decreases with an increase in degree of esterification. For pectins with different degrees of methylation, the extent of hydrolysis of PGI is increased with increasing of methoxyl content Aspergillus niger ?
-
 ?
3.2.1.82 pectin + H2O PGI activity on pectin decreases with an increase in degree of esterification. For pectins with different degrees of methylation, the extent of hydrolysis of PGI is increased with increasing of methoxyl content Aspergillus niger ATCC 9029 ?
-
 ?
3.2.1.82 polygalacturonic acid + H2O PGI Aspergillus niger ?
-
 ?
3.2.1.82 polygalacturonic acid + H2O PGI Aspergillus niger ATCC 9029 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 monomer 1 * 32000, SDS-PAGE Aspergillus niger
3.2.1.82 monomer 1 * 32000, SDS-PAGE, PGI Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exopolygalacturonase
-
 Aspergillus niger
3.2.1.67 PGI
-
 Aspergillus niger
3.2.1.67 polygalacturonase
-
 Aspergillus niger
3.2.1.82 exopolygalacturonase
-
 Aspergillus niger
3.2.1.82 PGI
-
 Aspergillus niger
3.2.1.82 PGII
-
 Aspergillus niger
3.2.1.82 polygalacturonase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 40
-
-
 Aspergillus niger
3.2.1.82 40
-
-
 Aspergillus niger

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.82 20 80 PGI Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 30
-
-
 Aspergillus niger
3.2.1.82 30
-
 PGI is stable up to 30°C Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.82 53.4
-
 Pectin PGI, 8.9% degree of methylation of pectin Aspergillus niger
3.2.1.82 85.4
-
 polygalacturonic acid PGI Aspergillus niger
3.2.1.82 122.8
-
 Pectin PGI, 7.8% degree of methylation of pectin Aspergillus niger
3.2.1.82 132.7
-
 Pectin PGI, 26% degree of esterification of pectin Aspergillus niger
3.2.1.82 133.5
-
 Pectin PGI, 6% degree of methylation of pectin Aspergillus niger
3.2.1.82 160.2
-
 Pectin PGI, 12% degree of methylation of pectin Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 5
-
-
 Aspergillus niger
3.2.1.82 5
-
 PGI in sodium acetate buffer Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 3.5 8
-
 Aspergillus niger
3.2.1.82 3.5 8 PGI Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.67 Aspergillus niger isoelectric focusing
-
 7.6
3.2.1.82 Aspergillus niger isoelectic focusing of PGI
-
 7.6