Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tanigawa, M.; Shinohara, T.; Saito, M.; Nishimura, K.; Hasegawa, Y.; Wakabayashi, S.; Ishizuka, M.; Nagata, Y.D
    D-Amino acid dehydrogenase from Helicobacter pylori NCTC 11637 (2010), Amino Acids, 38, 247-255.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.5.1 expression in Escherichia coli Helicobacter pylori
1.4.99.6 expressed in Escherichia coli BL21(DE3) cells Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.5.1 benzoate 0.1 mM, 98% inhibition Helicobacter pylori
1.4.5.1 iodoacetamide 0.1 mM, 66% inhibition Helicobacter pylori
1.4.5.1 iodoacetic acid 0.1 mM, 62% inhibition Helicobacter pylori
1.4.5.1 additional information EDTA, Mg2+ and Ca2+ have no effect on DadA activity Helicobacter pylori
1.4.5.1 p-hydroxybenzoic acid 0.1 mM, 79% inhibition Helicobacter pylori
1.4.99.6 4-hydroxybenzoic acid 21.4% residual activity at 0.1 mM Helicobacter pylori
1.4.99.6 benzoate 2% residual activity at 0.1 mM Helicobacter pylori
1.4.99.6 iodoacetamide 34.2% residual activity at 0.1 mM Helicobacter pylori
1.4.99.6 iodoacetate 38.2% residual activity at 0.1 mM Helicobacter pylori
1.4.99.6 additional information not inhibited by EDTA Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.5.1 0.0082
-
 oxidized coenzyme Q1 pH 8.0, 37°C Helicobacter pylori
1.4.5.1 40.2
-
 D-proline pH 8.0, 37°C Helicobacter pylori
1.4.99.6 0.0082
-
 coenzyme Q1 in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 40.2
-
 D-Pro with Q1 as the electron acceptor, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.5.1 additional information Mg2+ and Ca2+ have no effect on DadA activity Helicobacter pylori
1.4.99.6 additional information not influenced by Mg2+ and Ca+ Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.5.1 44000
-
 x * 44000, SDS-PAGE Helicobacter pylori
1.4.5.1 46040
-
 x * 46040, calculated from sequence Helicobacter pylori
1.4.99.6 44000
-
 x * 44000, SDS-PAGE Helicobacter pylori
1.4.99.6 46040
-
 x * 46040, calculated from amino acid sequence Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.99.6 D-Pro + H2O + coenzyme Q1 Helicobacter pylori D-Pro is the best substrate ?
-
 ?
1.4.99.6 D-Pro + H2O + coenzyme Q1 Helicobacter pylori NCTC 11637 D-Pro is the best substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.5.1 Helicobacter pylori A3KEZ1 NCTC 11637
-
1.4.99.6 Helicobacter pylori A3KEZ1
-
-
1.4.99.6 Helicobacter pylori NCTC 11637 A3KEZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.5.1 from Helicobacter pylori and recombinant Escherichia coli cells Helicobacter pylori
1.4.99.6 DEAE-Toyopearl column chromatography Helicobacter pylori

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.5.1 6.22
-
-
 Helicobacter pylori
1.4.99.6 0.05
-
 purified enzyme, with 20 mM D-Asp as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 0.05
-
 purified enzyme, with 20 mM D-Met as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 0.05
-
 purified enzyme, with 20 mM D-Val as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 0.06
-
 purified enzyme, with 20 mM D-Glu as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 0.12
-
 purified enzyme, with 20 mM L-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 0.22
-
 purified enzyme, with 20 mM L-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 0.46
-
 cell-free extract, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 1.25
-
 purified enzyme, with 20 mM D-Ser as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 2.29
-
 purified enzyme, with 20 mM D-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori
1.4.99.6 6.22
-
 purified enzyme, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37°C Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.5.1 D-Ala + oxidized coenzyme Q1 + H2O specific activity is 37% of that with D-proline Helicobacter pylori pyruvate + NH3 + reduced coenzyme Q1
-
 ?
1.4.5.1 D-Phe + oxidized coenzyme Q1 + H2O specific activity is 20% of that with D-proline Helicobacter pylori phenylpyruvate + NH3 + reduced coenzyme Q1
-
 ?
1.4.5.1 D-proline + oxidized coenzyme Q1 + H2O
-
 Helicobacter pylori DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
 ?
1.4.5.1 D-Ser + oxidized coenzyme Q1 + H2O specific activity is 7% of that with D-proline Helicobacter pylori 3-hydroxy-2-oxopropanoate + NH3 + reduced coenzyme Q1
-
 ?
1.4.5.1 additional information activity with D-Val, D-Met, D-Glu, D-Asp, L-Pro and L-Ala is below 5% of the activity with D-Pro Helicobacter pylori ?
-
 ?
1.4.99.6 D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol 36.8% activity compared to D-Pro Helicobacter pylori pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol 36.8% activity compared to D-Pro Helicobacter pylori NCTC 11637 pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori 2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori NCTC 11637 2-oxobutanedioate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol 1.0% activity compared to D-Pro Helicobacter pylori 2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol 1.0% activity compared to D-Pro Helicobacter pylori NCTC 11637 2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Met + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Met + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori NCTC 11637 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Phe + H2O + oxidized 2,6-dichlorophenolindophenol 19.8% activity compared to D-Pro Helicobacter pylori phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Pro + H2O + coenzyme Q1 D-Pro is the best substrate Helicobacter pylori ?
-
 ?
1.4.99.6 D-Pro + H2O + coenzyme Q1 D-Pro is the best substrate Helicobacter pylori NCTC 11637 ?
-
 ?
1.4.99.6 D-Pro + H2O + oxidized 2,6-dichlorophenolindophenol 100% activity Helicobacter pylori DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Ser + H2O + oxidized 2,6-dichlorophenolindophenol 6.7% activity compared to D-Pro Helicobacter pylori 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 D-Val + H2O + oxidized 2,6-dichlorophenolindophenol 0.8% activity compared to D-Pro Helicobacter pylori 2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
 ?
1.4.99.6 L-Ala + H2O + oxidized 2,6-dichlorophenolindophenol 1.9% activity compared to D-Pro Helicobacter pylori ?
-
 ?
1.4.99.6 L-Pro + H2O + oxidized 2,6-dichlorophenolindophenol 3.5% activity compared to D-Pro Helicobacter pylori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.5.1 ? x * 44000, SDS-PAGE Helicobacter pylori
1.4.5.1 ? x * 46040, calculated from sequence Helicobacter pylori
1.4.99.6 ? x * 44000, SDS-PAGE Helicobacter pylori
1.4.99.6 ? x * 46040, calculated from amino acid sequence Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
1.4.5.1 DadA
-
 Helicobacter pylori
1.4.99.6 D-amino acid dehydrogenase
-
 Helicobacter pylori
1.4.99.6 DAD
-
 Helicobacter pylori
1.4.99.6 DadA
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.5.1 37
-
-
 Helicobacter pylori
1.4.99.6 37
-
-
 Helicobacter pylori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.5.1 25 55 25°C: about 50% of maximal activity, 55°C: about 65% of maximal activity Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.5.1 8
-
-
 Helicobacter pylori
1.4.99.6 8
-
-
 Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.5.1 7.5 8.5 pH 7.5: about 50% of maximal activity, pH 8.5: about 45% of maximal activity Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.5.1 FAD flavoenzyme Helicobacter pylori
1.4.99.6 FAD
-
 Helicobacter pylori