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Literature summary extracted from

  • Kubota, K.; Nagata, K.; Miyazono, K.; Toyama, H.; Matsushita, K.; Tanokura, M.
    Purification, crystallization and preliminary X-ray analysis of L-sorbose reductase from Gluconobacter frateurii complexed with L-sorbose or NADPH (2009), Acta Crystallogr. Sect. F, 65, 562-564.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.289 into the HindIII/NotI site of pET-28a(+) plasmid, expression construct is designed to overexpress only L-sorbose reductase protein (residues 1-263), overexpressed in Escherichia coli BL21 (DE3) cells Gluconobacter frateurii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.289 in complex with L-sorbose or NADPH using the sitting-drop vapour-diffusion method, at 20°C, to 2.38 and 1.90 A resolution, respectively. Crystal of the L-sorbose reductase-L-sorbose complex belongs to space group C2221, with unit-cell parameters a=124.2, b=124.1, c=60.8 A. The crystal of the L-sorbose reductase-NADPH complex belongs to space group P21, with unit-cell parameters a=124.3, b=61.0, c=124.5A, beta= 89.99°. The crystals contain two and eight molecules, respectively, in the asymmetric unit Gluconobacter frateurii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.289 Gluconobacter frateurii A4PB64
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.289 by sonication, centrifugation and gel filtration Gluconobacter frateurii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.289 L-sorbose + NADPH + H+
-
 Gluconobacter frateurii L-sorbitol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.289 L-sorbose reductase
-
 Gluconobacter frateurii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.289 NADPH
-
 Gluconobacter frateurii