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Literature summary extracted from

  • Samra, Z.Q.; Athar, M.A.
    Cloning, sequence, expression and characterization of human beta-mannosidase (2008), Acta Biochim. Pol., 55, 479-490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.25
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.25 Ag+ 1.5 mM, no residual activity Homo sapiens
3.2.1.25 Co2+ 1.5 mM, 80% residual activity Homo sapiens
3.2.1.25 Cu2+ 1.5 mM, no residual activity Homo sapiens
3.2.1.25 dimethyl sulfoxide 5 mM, no residual activity Homo sapiens
3.2.1.25 dimethylformamide 5 mM, no residual activity Homo sapiens
3.2.1.25 ethanol 5 mM, no residual activity Homo sapiens
3.2.1.25 iodoacetate 5 mM, no residual activity Homo sapiens
3.2.1.25 Pb2+ 1.5 mM, 78% residual activity Homo sapiens
3.2.1.25 Sodium dodecyl sulfate 5 mM, no residual activity Homo sapiens
3.2.1.25 Zn2+ 1.5 mM, no residual activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.25 2.5 3 4-nitrophenyl-beta-D-mannopyranoside pH 5.0, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.25 97000
-
 x * 97000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.25 Homo sapiens A7LFP5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.25 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.25 additional information transcription in all cells and tissues tested Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.25 4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + D-mannopyranose
-
 ?
3.2.1.25 additional information no substrate: 4-nitrophenyl-alpha-D-mannose Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.25 ? x * 97000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.25 37
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.25 5
-
-
 Homo sapiens