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Literature summary extracted from

  • Ma, L.Y.; Chi, Z.M.; Li, J.; Wu, L.F.
    Overexpression of alginate lyase of Pseudoalteromonas elyakovii in Escherichia coli, purification, and characterization of the recombinant alginate lyase (2008), World J. Microbiol. Biotechnol., 24, 89-96.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.3 BaCl2
-
 Pseudoalteromonas elyakovii
4.2.2.3 CaCl2
-
 Pseudoalteromonas elyakovii
4.2.2.3 KCl
-
 Pseudoalteromonas elyakovii
4.2.2.3 MgCl2
-
 Pseudoalteromonas elyakovii
4.2.2.3 MnCl2
-
 Pseudoalteromonas elyakovii
4.2.2.3 NaCl
-
 Pseudoalteromonas elyakovii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 overexpressed in Escherichia coli Pseudoalteromonas elyakovii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 1,10-phenanthroline relative activity: 95% Pseudoalteromonas elyakovii
4.2.2.3 AgNO3 relative activity: 28% Pseudoalteromonas elyakovii
4.2.2.3 CoCl2 relative activity: 717 Pseudoalteromonas elyakovii
4.2.2.3 EDTA relative activity: 48% Pseudoalteromonas elyakovii
4.2.2.3 EGTA relative activity: 71% Pseudoalteromonas elyakovii
4.2.2.3 PMSF relative activity: 95% Pseudoalteromonas elyakovii
4.2.2.3 SDS
-
 Pseudoalteromonas elyakovii
4.2.2.3 ZnSO4 relative activity: 78% Pseudoalteromonas elyakovii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.3 BaCl2 stimulates activity, relative activity: 198% Pseudoalteromonas elyakovii
4.2.2.3 CaCl2 stimulates activity, relative activity: 176% Pseudoalteromonas elyakovii
4.2.2.3 KCl stimulates activity, relative activity: 109% Pseudoalteromonas elyakovii
4.2.2.3 MgCl2 stimulates activity, relative activity: 156% Pseudoalteromonas elyakovii
4.2.2.3 MnCl2 stimulates activity, relative activity: 121% Pseudoalteromonas elyakovii
4.2.2.3 NaCl stimulates activity, relative activity: 113% Pseudoalteromonas elyakovii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.3 32000
-
 SDS-PAGE Pseudoalteromonas elyakovii

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Pseudoalteromonas elyakovii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 using gel filtraton and anion-exchange chromatography Pseudoalteromonas elyakovii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Pseudoalteromonas elyakovii unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 poly alpha-L-guluronate
-
 Pseudoalteromonas elyakovii oligo-alpha-1,4-L-guluronate
-
 ?
4.2.2.3 poly beta-D-mannuronate
-
 Pseudoalteromonas elyakovii oligo-beta-1,4-D-mannuronate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 alginate lyase
-
 Pseudoalteromonas elyakovii
4.2.2.3 alyPEEC
-
 Pseudoalteromonas elyakovii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 30
-
-
 Pseudoalteromonas elyakovii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3 70
-
 enzyme totally inactivated at 70°C within 10 min Pseudoalteromonas elyakovii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 7
-
-
 Pseudoalteromonas elyakovii
4.2.2.3 7.5
-
 assay at Pseudoalteromonas elyakovii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 6 7
-
 Pseudoalteromonas elyakovii