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Literature summary extracted from

  • Wang, Y.; Zhang, X.
    Identification and characterization of a novel thymidylate synthase from deep-sea thermophilic bacteriophage Geobacillus virus E2 (2008), Virus Genes, 37, 218-224.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expression in Escherichia coli as fusion protein with glutathione S-transferase Geobacillus virus E2
2.1.1.45 expression in Escherichia coli BL21 (DE3) as a fusion protein with glutathione S-transferase (GST) Geobacillus virus E2

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.45 31000
-
recombinant GVE2-TS protein, SDS-PAGE Geobacillus virus E2
2.1.1.45 31000
-
x * 31000, SDS-PAGE and calculated Geobacillus virus E2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Geobacillus virus E2 thymidylate synthase is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms 7,8-dihydrofolate + dTMP
-
?
2.1.1.45 additional information Geobacillus virus E2 recombinant protein is able to bind its own RNA ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Geobacillus virus E2
-
GVE2, deep-sea thermophilic bacteriophage
-
2.1.1.45 Geobacillus virus E2 A6M9A2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.45 GST-GVE2 TS fusion protein is purified by glutathione-Sepharose beads Geobacillus virus E2

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 additional information expressed early after Geobacillus virus E2 infection Geobacillus virus E2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
Geobacillus virus E2 7,8-dihydrofolate + dTMP
-
?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP thymidylate synthase is essential for de novo synthesis of dTMP and is a key enzyme involved in DNA synthesis and transcriptional regulation of organisms Geobacillus virus E2 7,8-dihydrofolate + dTMP
-
?
2.1.1.45 additional information recombinant protein is able to bind its own RNA Geobacillus virus E2 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.45 ? x * 31000, SDS-PAGE and calculated Geobacillus virus E2

Synonyms

EC Number Synonyms Comment Organism
2.1.1.45 Thy1
-
Geobacillus virus E2
2.1.1.45 ThyA
-
Geobacillus virus E2