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Literature summary extracted from

  • Dunn, M.F.; Niks, D.; Ngo, H.; Barends, T.R.; Schlichting, I.
    Tryptophan synthase: the workings of a channeling nanomachine (2008), Trends Biochem. Sci., 33, 254-264.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.8 D60N site-directed mutagenesis, substitution of asparagine for aspartic acid in the alphaAsp60Asn variant. alphaAsp60 has an essential role in stabilizing charge development on the IGP indole ring during C-C bond scission Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 T183V site-directed mutagenesis, substitution of valine for threonine in the alphaThr183Val variant, resulting in impaired catalysis at the alpha-site and loss of allosteric communication Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.8 2,3-dihydroxy-3-[(2-hydroxyphenyl)amino] propyl phosphate competetive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 2-([[4-(trifluoromethoxy)phenyl]sulfonyl]amino) ethyl phosphate competetive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 3-(1H-indol-3-yl)propyl phosphate competetive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 3-(2,3-dihydro-1H-indol-3-yl)glycerol phosphate competetive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 3-acetyl-L-aspartic acid inhibits the alpha-reaction. Indole 3-acetylglycine and 3-acetyl-L-aspartic acid are similar to complexes that are similar to 3-(1H-indol-3-yl)propyl phosphate with the phosphoryl sub-site occupied by the ligand carboxylate, the indole ring NH H-bonded to alphaAsp60, and one carboxylate oxygen of the catalytic alphaGlu49 H-bonded to the acetyl oxygen Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 4-[(2-aminophenyl)sulfonyl]butyl phosphate competetive inhibitor Salmonella enterica subsp. enterica serovar Typhimurium
4.1.2.8 indole 3-acetylglycine inhibits the alpha-reaction. Indole 3-acetylglycine and 3-acetyl-L-aspartic acid are similar to complexes that are similar to 3-(1H-indol-3-yl)propyl phosphate with the phosphoryl sub-site occupied by the ligand carboxylate, the indole ring NH H-bonded to alphaAsp60, and one carboxylate oxygen of the catalytic alphaGlu49 H-bonded to the acetyl oxygen Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.8 Salmonella enterica subsp. enterica serovar Typhimurium
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.8 indole-3-glycerol phosphate
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 Salmonella enterica subsp. enterica serovar Typhimurium indole + D-glyceraldehyde 3-phosphate
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 ?