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Literature summary extracted from

  • de Santi Ferrara, G.I.; Fernandes-Pedrosa, M.D.; Junqueira-de-Azevedo, I.D.; Goncalves-de-Andrade, R.M.; Portaro, F.C.; Manzoni-de-Almeida, D.; Murakami, M.T.; Arni, R.K.; van den Berg, C.W.; Ho, P.L.; Tambourgi, D.V.
    SMase II, a new sphingomyelinase D from Loxosceles laeta venom gland: Molecular cloning, expression, function and structural analysis (2009), Toxicon, 53, 743-753.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 expressed in Escherichia coli Loxosceles laeta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.41 Mg2+ contains Mg2+ Loxosceles laeta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.41 35000
-
 His6-tagged protein Smase II, SDS-PAGE Loxosceles laeta

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Loxosceles laeta Q8I912
-
-
3.1.4.41 Loxosceles laeta Q8I914
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.41 Ni2+-chelating Sepharose column chromatography Loxosceles laeta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.41 venom
-
 Loxosceles laeta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles laeta ceramide phosphate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 SMase D
-
 Loxosceles laeta
3.1.4.41 Smase D 1
-
 Loxosceles laeta
3.1.4.41 SMase I recombinant enzyme Loxosceles laeta
3.1.4.41 SMase II recombinant enzyme Loxosceles laeta
3.1.4.41 sphingomyelinase D
-
 Loxosceles laeta
3.1.4.41 sphingomyelinase D 1
-
 Loxosceles laeta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.41 0.004
-
 sphingomyelin crude enzyme, in HEPES-buffered saline at 37°C Loxosceles laeta
3.1.4.41 0.0065
-
 sphingomyelin recombinant enzyme SMase I, in HEPES-buffered saline at 37°C Loxosceles laeta
3.1.4.41 0.0175
-
 sphingomyelin recombinant enzyme SMase II, in HEPES-buffered saline at 37°C Loxosceles laeta