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Literature summary extracted from

  • Tokunaga, H.; Arakawa, T.; Tokunaga, M.
    Engineering of halophilic enzymes: two acidic amino acid residues at the carboxy-terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases (2008), Protein Sci., 17, 1603-1610.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 A134E/A135E site-directed mutagenesis, the mutant shows decreased oligomerization compared to the wild-type enzyme Pseudomonas sp.
2.7.4.6 E134A/E135A site-directed mutagenesis, the mutant shows increased oligomerization compared to the wild-type enzyme Halomonas sp. #593

General Stability

EC Number General Stability Organism
2.7.4.6 NaCl stabilizes the A134E/A135E mutant enzyme, but not the wild-type enzyme Pseudomonas sp.
2.7.4.6 NaCl stabilizes the wild-type enzyme, but the E134A/E135A mutant Halomonas sp. #593

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 additional information Halomonas sp. #593 critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Halomonas sp. #593 Q83WH5
-
-
2.7.4.6 Pseudomonas sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.6 1000
-
 about, in absence of NaCl, purified wild-type enzyme Halomonas sp. #593
2.7.4.6 1100
-
 about, in absence of NaCl, purified wild-type enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 additional information critical role of the two amino acid residues, E134 and E135, at the carboxy-terminal region of NDK in determining the halophilic characteristics of the protein, overview Halomonas sp. #593 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 dimer the sequence of amino acids A-Y-F-F-E-E-S-E of HaNDK is involved in the tetrameric assembly Halomonas sp. #593
2.7.4.6 tetramer the sequence of amino acids A-Y-F-F-AA-T-E of PaNDK is involved in the tetrameric assembly Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.6 30
-
 assay at Pseudomonas sp.
2.7.4.6 30
-
 assay at Halomonas sp. #593

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.6 85
-
 5 min, the enzyme shows irreversible heat denaturation Pseudomonas sp.
2.7.4.6 85
-
 5 min, the enzyme shows reversible heat denaturation Halomonas sp. #593