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Literature summary extracted from

  • Hancock, A.N.; Coleman, R.S.; Johnson, R.T.; Sarisky, C.A.; Johann, T.W.
    Investigations of the roles of arginine 115 and lysine 120 in the active site of 5,10-methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (2008), Protein J., 27, 303-308.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.3.2 wild type and mutant enzymes are expressed in Escherichia coli strain BL21(DE3)/pSJS1244 Mycoplasmoides pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
6.3.3.2 K120A the mutant shows increased Km and turnover number values for ATP and 5-formyltetrahydrofolate compared to the wild type enzyme Mycoplasmoides pneumoniae
6.3.3.2 R115A the mutant shows no activity Mycoplasmoides pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.2 0.0025
-
 5-formyltetrahydrofolate wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae
6.3.3.2 0.0071
-
 5-formyltetrahydrofolate mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae
6.3.3.2 0.076
-
 ATP wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae
6.3.3.2 1.2
-
 ATP mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.2 Mycoplasmoides pneumoniae P75430
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.2 Talon metal affinity resin chromatography and Sephadex G-25 gel filtration Mycoplasmoides pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.2 ATP + 5-formyltetrahydrofolate
-
 Mycoplasmoides pneumoniae ADP + 5,10-methenyltetrahydrofolate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.3.2 5,10-Methenyltetrahydrofolate synthetase
-
 Mycoplasmoides pneumoniae
6.3.3.2 MTHFS
-
 Mycoplasmoides pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.3.2 0.79
-
 5-formyltetrahydrofolate wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae
6.3.3.2 1
-
 ATP wild type enzyme, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae
6.3.3.2 1.3
-
 5-formyltetrahydrofolate mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae
6.3.3.2 1.7
-
 ATP mutant enzyme K120A, in 200 mM MES pH 6.0, with 30 mM NaCl, 10 mM MgCl2, 0.1% (v/v) Triton X-100, and 0.01% (v/v) 2-mercaptoethanol, at 37°C Mycoplasmoides pneumoniae