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Literature summary extracted from

  • Lin, X.; Xu, S.; Yang, Y.; Wu, J.; Wang, H.; Shen, H.; Wang, H.
    Purification and characterization of anthranilate synthase component I (TrpE) from Mycobacterium tuberculosis H37Rv (2009), Protein Expr. Purif., 64, 8-15.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.3.27 medicine TrpE is a promising target for the design of novel anti-tuberculosis drugs Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.27 into the vector pET30a for expression in Escherichia coli BL21DE3 cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-tryptophan
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.27 Mg2+
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 62300
-
 recombinant fusion protein, determined by SDS-PAGE Mycobacterium tuberculosis
4.1.3.27 62350
-
 determined by mass spectrometry Mycobacterium tuberculosis
4.1.3.27 62370
-
 calculated Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.27 chorismate + ammonia Mycobacterium tuberculosis
-
 anthranilate + pyruvate + H2O
-
 ?
4.1.3.27 chorismate + ammonia Mycobacterium tuberculosis H37Rv
-
 anthranilate + pyruvate + H2O
-
 ?
4.1.3.27 chorismate + L-glutamine Mycobacterium tuberculosis
-
 anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-glutamine Mycobacterium tuberculosis H37Rv
-
 anthranilate + pyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Mycobacterium tuberculosis P9WFX3
-
-
4.1.3.27 Mycobacterium tuberculosis H37Rv P9WFX3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 using Ni-NTA His-binding resin, the 6xHis-tag is removed by digestion with enterokinase Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.27 2.16
-
 crude supernatant Mycobacterium tuberculosis
4.1.3.27 4.44
-
 purification step Ni-NTA column Mycobacterium tuberculosis
4.1.3.27 5.2
-
 after concentration step Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + ammonia
-
 Mycobacterium tuberculosis anthranilate + pyruvate + H2O
-
 ?
4.1.3.27 chorismate + ammonia
-
 Mycobacterium tuberculosis H37Rv anthranilate + pyruvate + H2O
-
 ?
4.1.3.27 chorismate + L-glutamine
-
 Mycobacterium tuberculosis anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + L-glutamine
-
 Mycobacterium tuberculosis H37Rv anthranilate + pyruvate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.27 anthranilate synthase component I
-
 Mycobacterium tuberculosis
4.1.3.27 TrpE
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.27 30
-
 activity assay Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.3.27 30 37 incubation at temperatures higher than 37°C results in a significant reduction of activity Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 9
-
 activity assay Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.27 7 10 pH 7 20% relative activity, pH 10 60% relative activity Mycobacterium tuberculosis