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Literature summary extracted from

  • Bohlmann, J.; Crock, J.; Jetter, R.; Croteau, R.
    Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-alpha-bisabolene synthase from grand fir (Abies grandis) (1998), Proc. Natl. Acad. Sci. USA, 95, 6756-6761.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.38 additional information the enzyme is wound-inducible Abies grandis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.38 gene ag1, cloning from a wound-induced stem-cDNA library, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, functional expression in Escherichia coli strain XL1-Blue Abies grandis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.38 Mn2+ activates, but Mn2+ at concentrations higher than 1 mM results in a decline of activity with either substrate Abies grandis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.38 KCl only weakly influences GDP conversion with the ag1 enzyme causing a 2fold activation at 100 mM KCl, but the monovalent cation has no effect with FDP as substrate Abies grandis
4.2.3.38 Mg2+ activates, saturation with Mg2+ is reached at 5 mM, and no apparent inhibition of catalysis occurs up to 100 mM Abies grandis
4.2.3.38 Mn2+ activates, but Mn2+ at concentrations higher than 1 mM results in a decline of activity with either substrate Abies grandis
4.2.3.38 additional information the activity of recombinant ag1 requires a divalent cation cofactor, Mg2+ or Mn2+, which is employed to neutralize the negative charge of the diphosphate leaving group in the substrate ionization step of the reaction sequence. Mg2+ is more efficient in catalysis than is Mn2+. With GDP as substrate, however, Mn2+ at 0.5 mM yields a 4fold higher rate of monoterpene synthase activity compared to Mg2+ at concentrations up to 50 mM Abies grandis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.38 93776
-
 x * 93776, sequence calculation Abies grandis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.38 (2E,6E)-farnesyl diphosphate Abies grandis
-
 (E)-alpha-bisabolene + diphosphate (E)-alpha-bisabolene is the precursor in Abies species of todomatuic acid, juvabione, and related insect juvenile hormone mimics, overview ?
4.2.3.38 additional information Abies grandis induced (E)-alpha-bisabolene biosynthesis constitutes part of a defense response targeted to insect herbivores, and possibly fungal pathogens, that is distinct from induced oleoresin monoterpene production ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.38 Abies grandis O81086 gene ag1
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.38 (2E,6E)-farnesyl diphosphate = (E)-alpha-bisabolene + diphosphate electrophilic reaction mechanism Abies grandis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.38 (2E,6E)-farnesyl diphosphate
-
 Abies grandis (E)-alpha-bisabolene + diphosphate (E)-alpha-bisabolene is the precursor in Abies species of todomatuic acid, juvabione, and related insect juvenile hormone mimics, overview ?
4.2.3.38 (2E,6E)-farnesyl diphosphate the recombinant enzymes is substrate-specific and produces (E)-alpha-bisabolene as sole product Abies grandis (E)-alpha-bisabolene + diphosphate
-
 ?
4.2.3.38 additional information induced (E)-alpha-bisabolene biosynthesis constitutes part of a defense response targeted to insect herbivores, and possibly fungal pathogens, that is distinct from induced oleoresin monoterpene production Abies grandis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.38 ? x * 93776, sequence calculation Abies grandis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.38 (E)-alpha-bisabolene synthase
-
 Abies grandis
4.2.3.38 More the enzyme belongs to the terpenoid synthases, subgroup sesquiterpene synthases Abies grandis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.38 25
-
 assay at Abies grandis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.38 Abies grandis sequence calculation
-
 5.03