Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sirikantaramas, S.; Yamazaki, M.; Saito, K.
    Mutations in topoisomerase I as a self-resistance mechanism coevolved with the production of the anticancer alkaloid camptothecin in plants (2008), Proc. Natl. Acad. Sci. USA, 105, 6782-6786.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.1 D-galactose
-
Ophiorrhiza pumila
5.6.2.1 D-galactose
-
Camptotheca acuminata
5.6.2.1 D-galactose
-
Homo sapiens
5.6.2.1 D-galactose
-
Ophiorrhiza liukiuensis
5.6.2.1 D-galactose
-
Ophiorrhiza japonica
5.6.2.1 D-galactose
-
Arabidopsis thaliana
5.6.2.1 D-glucose
-
Ophiorrhiza pumila
5.6.2.1 D-glucose
-
Camptotheca acuminata
5.6.2.1 D-glucose
-
Homo sapiens
5.6.2.1 D-glucose
-
Ophiorrhiza liukiuensis
5.6.2.1 D-glucose
-
Ophiorrhiza japonica
5.6.2.1 D-glucose
-
Arabidopsis thaliana

Application

EC Number Application Comment Organism
5.6.2.1 drug development comparison of the target sites in plants exploited for obtaining additional information on drug-target interaction and for designing advanced drugs to prevent resistance in cancer cells and combat drug resistance Catharanthus roseus
5.6.2.1 drug development comparison of the target sites in plants exploited for obtaining additional information on drug-target interaction and for designing advanced drugs to prevent resistance in cancer cells and combat drug resistance Ophiorrhiza pumila
5.6.2.1 drug development comparison of the target sites in plants exploited for obtaining additional information on drug-target interaction and for designing advanced drugs to prevent resistance in cancer cells and combat drug resistance Camptotheca acuminata
5.6.2.1 drug development comparison of the target sites in plants exploited for obtaining additional information on drug-target interaction and for designing advanced drugs to prevent resistance in cancer cells and combat drug resistance Ophiorrhiza liukiuensis
5.6.2.1 drug development comparison of the target sites in plants exploited for obtaining additional information on drug-target interaction and for designing advanced drugs to prevent resistance in cancer cells and combat drug resistance Ophiorrhiza japonica
5.6.2.1 drug development comparison of the target sites in plants exploited for obtaining additional information on drug-target interaction and for designing advanced drugs to prevent resistance in cancer cells and combat drug resistance Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.1 expressed in Saccharomyces cerevisiae Catharanthus roseus
5.6.2.1 expressed in Saccharomyces cerevisiae Ophiorrhiza japonica
5.6.2.1 expressed in Saccharomyces cerevisiae Arabidopsis thaliana
5.6.2.1 heterologously expressed in Escherichia coli exhibits topoisomerase activity Ophiorrhiza pumila

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.1 I420V prepared by PCR-based mutagenesis Ophiorrhiza japonica
5.6.2.1 L530I prepared by PCR-based mutagenesis Ophiorrhiza japonica
5.6.2.1 N421K prepared by PCR-based mutagenesis Ophiorrhiza japonica
5.6.2.1 N722S prepared by PCR-based mutagenesis Ophiorrhiza japonica

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 camptothecin
-
Arabidopsis thaliana
5.6.2.1 camptothecin
-
Camptotheca acuminata
5.6.2.1 camptothecin
-
Catharanthus roseus
5.6.2.1 camptothecin
-
Homo sapiens
5.6.2.1 camptothecin
-
Ophiorrhiza japonica
5.6.2.1 camptothecin
-
Ophiorrhiza liukiuensis
5.6.2.1 camptothecin
-
Ophiorrhiza pumila

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Arabidopsis thaliana P30181
-
-
5.6.2.1 Camptotheca acuminata
-
camptothecin producing plant
-
5.6.2.1 Catharanthus roseus
-
camptothecin non producing plant
-
5.6.2.1 Homo sapiens P11387
-
-
5.6.2.1 Ophiorrhiza japonica
-
camptothecin non producing plant
-
5.6.2.1 Ophiorrhiza liukiuensis
-
camptothecin producing plant has Top1s with point mutations that confer resistance to camptothecin, suggesting the effect of an endogenous toxic metabolite on the evolution of the target cellular component
-
5.6.2.1 Ophiorrhiza pumila
-
camptothecin producing plant
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.1 hairy root
-
Ophiorrhiza pumila
-
5.6.2.1 hairy root
-
Ophiorrhiza liukiuensis
-
5.6.2.1 leaf young Catharanthus roseus
-
5.6.2.1 leaf young Camptotheca acuminata
-
5.6.2.1 leaf young Ophiorrhiza japonica
-

Synonyms

EC Number Synonyms Comment Organism
5.6.2.1 AtTop1
-
Arabidopsis thaliana
5.6.2.1 Ca-Top1
-
Camptotheca acuminata
5.6.2.1 CrTop1
-
Catharanthus roseus
5.6.2.1 HsTop1
-
Homo sapiens
5.6.2.1 human Top1
-
Homo sapiens
5.6.2.1 OjTop1
-
Ophiorrhiza japonica
5.6.2.1 OlTop1
-
Ophiorrhiza liukiuensis
5.6.2.1 OpTop1
-
Ophiorrhiza pumila
5.6.2.1 Top1
-
Catharanthus roseus
5.6.2.1 Top1
-
Ophiorrhiza pumila
5.6.2.1 Top1
-
Camptotheca acuminata
5.6.2.1 Top1
-
Homo sapiens
5.6.2.1 Top1
-
Ophiorrhiza liukiuensis
5.6.2.1 Top1
-
Ophiorrhiza japonica
5.6.2.1 Top1
-
Arabidopsis thaliana
5.6.2.1 Topoisomerase I
-
Catharanthus roseus
5.6.2.1 Topoisomerase I
-
Ophiorrhiza pumila
5.6.2.1 Topoisomerase I
-
Camptotheca acuminata
5.6.2.1 Topoisomerase I
-
Homo sapiens
5.6.2.1 Topoisomerase I
-
Ophiorrhiza liukiuensis
5.6.2.1 Topoisomerase I
-
Ophiorrhiza japonica
5.6.2.1 Topoisomerase I
-
Arabidopsis thaliana