EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.7 | expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.7 | H281A | mutant with 152fold decreased kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H281F | mutant with 4.9fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H281N | mutant with 17fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H281Q | mutant with 37fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H281T | mutant with 159fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H281W | mutant with 19fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H281Y | mutant with 46fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H70A | mutant exhibits 4000fold decreased catalytic activity compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H70F | mutant exhibits a 236fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H70L | mutant exhibits a 33fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H70S | mutant exhibits a 197fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | H70T | mutant exhibits a 25fold decrease in kcat/Km compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | S26A | mutant with 21fold decrease in kcat value compared to the wild type enzyme | Pseudomonas putida |
4.1.1.7 | S26L | mutant exhibits no significant loss of activity compared to the wild type enzyme (2fold decrease in kcat value) | Pseudomonas putida |
4.1.1.7 | S26M | mutant exhibits no significant loss of activity compared to the wild type enzyme (12fold decrease in kcat value) | Pseudomonas putida |
4.1.1.7 | S26T | mutant exhibits no significant loss of activity compared to the wild type enzyme (3fold decrease in kcat value) | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 0.27 | - |
benzoylformate | wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.29 | - |
benzoylformate | mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.29 | - |
benzoylformate | mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.38 | - |
benzoylformate | mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.49 | - |
benzoylformate | mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.54 | - |
benzoylformate | mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.8 | - |
benzoylformate | mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.85 | - |
benzoylformate | mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.85 | - |
benzoylformate | mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.89 | - |
benzoylformate | mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.9 | - |
benzoylformate | mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 0.94 | - |
benzoylformate | mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 1.2 | - |
benzoylformate | mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 1.2 | - |
benzoylformate | mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 1.5 | - |
benzoylformate | mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 2.9 | - |
benzoylformate | mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 7.8 | - |
benzoylformate | mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.7 | Pseudomonas putida | P20906 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.7 | - |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.7 | benzoylformate | - |
Pseudomonas putida | benzaldehyde + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.7 | BFDC | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.7 | 0.46 | - |
benzoylformate | mutant enzyme H70A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 2 | - |
benzoylformate | mutant enzyme H281T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 2.1 | - |
benzoylformate | mutant enzyme H281A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 4.5 | - |
benzoylformate | mutant enzyme H70F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 5.6 | - |
benzoylformate | mutant enzyme H70S, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 6.9 | - |
benzoylformate | mutant enzyme H281Y, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 8.6 | - |
benzoylformate | mutant enzyme H281Q, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 14 | - |
benzoylformate | mutant enzyme H70L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 15 | - |
benzoylformate | mutant enzyme S26A, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 17 | - |
benzoylformate | mutant enzyme H281W, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 19 | - |
benzoylformate | mutant enzyme H281N in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 26 | - |
benzoylformate | mutant enzyme S26M, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 42 | - |
benzoylformate | mutant enzyme H70T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 65 | - |
benzoylformate | mutant enzyme H281F, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 94 | - |
benzoylformate | mutant enzyme S26T, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 132 | - |
benzoylformate | mutant enzyme S26L, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida | |
4.1.1.7 | 320 | - |
benzoylformate | wild type enzyme, in 100 mM potassium phosphate buffer (pH 6.0), at 30°C | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.7 | thiamine diphosphate | 0.5 mM, required for activity | Pseudomonas putida |